| Project/Area Number |
61480458
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
物質生物化学
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
NATORI Shunji Faculty of Pharmaceutical Sciences, University of Tokyo,Professor, 薬学部, 教授 (50012662)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
UENO Kohji Faculty of Pharmaceutical Sciences, University of Tokyo,Assistant Professor, 薬学部, 助手 (10143504)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Project Status |
Completed (Fiscal Year 1987)
|
| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥4,000,000 (Direct Cost: ¥4,000,000)
|
|---|
| Keywords | Sarcophaga lectin / macrophages / receptor / membrane protein / Ehrlich ascites tumor cells / J774.1 cells / モノクローナル抗体 / センチニクバエレクチン / J774.1 |
|---|
| Research Abstract |
The structure of Sarcophaga lectin receptor on the surface of murine macrophages was analyzed using monoclonal antibodies. This receptor was found by gel filteration to have a molecular weight of 460 kDa. SDS-polyacrylamide gel electrophoresis showed that this receptor consists of two subunits of 170 kDa and 110 kDa Results indicated that it is probably a heterotetramer of two molecules of each subunit.
It was shown that the level of cyclic AMP decreases significantly, on treatment of murine macrophages with this lectin. Therefore, it was suggested that G-protein is involved in the process of the activation of macrophages by Sarcophaga lectin.
|
