| Project/Area Number |
61480471
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | University of Tokyo |
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Principal Investigator |
OISHI Michio Univ. of Tokyo, Inst. of Applied Microbiology,professor, 応用微生物研究所, 教授 (00126004)
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| Co-Investigator(Kenkyū-buntansha) |
NSHIMORI Katsuhiko Univ. of Tokyo, Inst. of Applied Microbiol., Assistant, 応用微生物研究所, 助手 (10164609)
NOMURA Shintaro Univ. of Tokyo, Inst. of Applied Microbiol., Assistant, 応用微生物研究所, 助手 (80159087)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Keywords | Differntiation / cell fusion / erythroid cells / プラスミド |
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| Research Abstract |
In order to identify intracellular factors involved in differentiation of mouse Friend cells, the differentiation process was first analyzed by cell fusion experiments. The experiments suggested that the process is a synergistic results of two basically different inducible intracellular reactions. One reaction is induced following damage to DNA and the other reaction is induced by typical erythroid inducing agents such as DMSO or HMBA. We searched for the putative transacting differentiation inducing factors and detected two proteinaceous factors (DIF-i and DIF-II) corresponding to these reactions. The partially purified factors triggered erythroid differentiation when introduced into undifferentiated cells, provided that the reciprent cells had been potentiated by the induction of either one of the two reactions. Mechanism of differentiation fo embryonal carcinoma cells was also studied.
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