| Project/Area Number |
61480477
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Okayama University |
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Principal Investigator |
KAGAWA HIROAKI Associate Professor, 理学部, 助教授 (10022732)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | Caenorhabditis elegans / paramyosin / muscle protein / exon-expression cloning / monoclonal antibody / invertebrate / coiled-coil structure / モノクロナール抗体 / 塩基配列決定 / 蛋白質構造 |
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| Research Abstract |
Nucleotide sequence of the paramyosin gene of Caenorhabditis elegans was determined by using shot gun procedure of Sanger method. The accession number in the EMBL/GenBak/DDBJ Nucleotide Sequence Databases was X08068. The gene was separated into 10 exons which encode 866 amino acid residues having molecular mass of 99,890 daltons. Amino acid sequence promised alpha-helical-coil-coil structure of the protein. Potential charge-mediated interactions between paramyosin and between paramyosin and myosinrod were caluculated using a model. Antigenic sites of the protein against antiparamyosin antiserum and three monoclonal antibodies were identified by immunological assay of fusion proteins which were produced in bacterial cells. The result could be useful for understanding molecular assembly of thick filament in invertebrates.
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