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Development A New Pure Culture Method without Heat-Sterilization and Air-Filtration Processes

Research Project

Project/Area Number 61490005
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionTsukuba University

Principal Investigator

HANAKA Hideo  Tsukuba University Institute of Applied Biochemistry, 応用生物化学系, 助教授 (40015657)

Co-Investigator(Kenkyū-buntansha) OHMOMO Sadahiro  Tsukuba University Institute of Applied Biochemistry, 応用生物化学系, 講師 (10015901)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1986: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsPure culture method / Anti-microbial substaces / Immobilized cell system / Toxic solvent Heat-sterilization process / Air-filtration process / Infection / 殺菌および除菌工程 / アルコール醗酵 / 抗微生物物質に対する防御機能 / 抗微生物性物質 / バイオリアタター
Research Abstract

A new immobilized cell system providing protection against toxic solvents such as 2-octanol and toluene was investigated so that normal fermentations could be carried out in a medium containing toxic solvents. The system consists of immobilized growing cells in Ca-alginate gel beads to which vegetable oils such as caster, soy bean, olive and tung oils, which are inexpensive absorbents of solvents, had been added. The system's protective function against a toxic solvent was related to the distribution coefficient of the solvent between a vegetable oil and water. The intensity of the function was larger as the coefficient of the solvent was larger, namely, the equilibrium concentration of the solvent in water was lower.
The new immobilized cell system, also, could provide excellent protection against oil-soluble antimicrobial substances as well as toxic solvents. A new pure culture method without heat-sterilization and air-filtration processes using the new immobilized cell system was investigated. The ethanol fermentation of Saccharomyces cerevisiae and the lactic acid fermentation of Streptococcus lactis were used as model fermentations to develop the new pure culture method. With the new immobilized growing cell systems using caster oil, continuous cultures for both fermentations without heat-sterilization and air-filtration processes were carried out in presence of oil-soluble antimicrobial substances. p-Hydroxy benzoate butyl (POBB), one of food preservatives, and Preventol GD, one of phenol disinfectants, were used as oil-soluble antimicrobial substances. In the case of POBB, continuous cultures for both fermentations could be normally carried out without infection for about 60 h, and in the case of Priventol GD, the continuous culture for ethanol fermentation could be similarly carried out for more than 140 h.
Therefore, a new pure culture method without heat-sterilization and airfiltration processes was developed.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 田中秀夫: Biotechnol.Bioeng.30. 22-30 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 田中秀夫: Biotechnol.Bioeng.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hideo Tanaka; Satoru Harada; Hiroshi Kurosawa; Mizuo Yajima: "A New Immobilized Cell System with Protection against Toxic Solvents." Biotechnology and Bioengineering. 30. 22-30 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hideo Tanaka; Tohru Ohta; Satoru Harada; Tomoyasu Nakajima: "Development of A New Culture Method without Heat-Sterilization and Air Filtration Processes." Biotechnology and Bioengineering 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 田中秀夫: Biotechuol.Bioeng.

    • Related Report
      1988 Annual Research Report
  • [Publications] 田中秀夫: Biotechnol.Bioeng.30. 22-30 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 田中秀夫: Bitechnol.Bioeng. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 田中秀夫: Biotechnol Bioeng. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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