• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structural Analysis of the Multigene Family in the Human MHC Class II Gene Region

Research Project

Project/Area Number 61540460
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionOkazaki National Research Institutes

Principal Investigator

OKADA Kiyotaka  National Institute for Basic Biology,Research Associate., 基礎生物学研究所・細胞生物学研究系細胞情報, 文部教官助手 (50101093)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsMHC class II genes / multigene family / polymorphism / repetitive sequence / chromosome walking / 主要組織適合抗原 / クロモソームウォーキング / 遺伝子クローニング / HLA遺伝子 / クラス【II】抗原 / 多重遺伝子 / クローニング
Research Abstract

Human MHC (Major Histocompatibility Complex) class II gene region is on the short arm of the 6th chromosome.More than 14 class II genes (6 <alpha>-chain genes and 8 <beta>chain genes) are clustered as <alpha>-<beta>gene pair in this region.Most of the genes are highly polymorphic in the human population.Immunological functions of the gene products are known to be differentiated.
This project aims to investigate the fine structure of the MHC class II multigene region and rationalize the possible mechanisms to generate this huge gene cluster accompanied by functional differentiation and high degree of polymorphism. Using the cosmid clones,I analyzed the organization of the gene pairs,and compared the homology between exons,introns and intergenic spaces,with several DNA probes including the Alu and kpn repetitive sequences. From these analyses, a model which involves gene duplications accompanied with some gene loss was proposed.
In order to investigate the functional difference between the class I antigens, cloned gene pairs'were transfered into mouse L cells, and assayed the stimulating. activities for IgG production and for T-cell activation (MLR).The DO gene products was shown to be different from the DR and DP gene products.Structural basis of the altered function of the DO gene pair was discussed.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 岡田清孝: 実験医学. 4. 394-400 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岡田清孝: 細胞工学. 5. 707-716 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岡田清孝: 蛋白質核酸酵素. 31. 1548-1564 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岡田清孝: 臨床免疫. 19. 285-292 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岡田清孝,菊地,矢田,奥村編: "Annual Review免疫1987" 中外医学社, 417 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岡田清孝,辻公美編: "HLAハンドブック" サイエンスフォーラム社, 347 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Okada,Kiyotaka: "Genetic Regulation of the HLA antigens." Jikken Igaku (in Japanese). 4. 394-400 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Okada,Kiuotaka: "Gene Structure of the HLA." Saibokogaku (in Japanese). 5. 707716 (1086)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Okada,Kiyotaka: "Structure and Function of the Major Histocompatibillity Complex Gene Cluster." TanpakusitsuKakusanKoso (Protein Nucleic Acid and Enzyme). 31. 1548-1564 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Okada,Kiyotaka.: "Polymorphism of the Human MHC class II genes." RinshoIgaku (in Japanese). 19. 285-292 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Okada,Kiyotaka: Chugai-Igakusha. Annual Review Meneki 1987, 124-135 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Okada,Kyotaka: Science Forum Ltd.HLA handbook, 57-73 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岡田清孝: 実験医学. 4. 394-400 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田清孝: 細胞工学. 5. 707-716 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田清孝: 蛋白質核酸酵素. 31. 1548-1564 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田清孝: "Annual Review 免疫1987" 中外医学社, 417 (1987)

    • Related Report
      1986 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi