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Structure and function of photosynthetic oxygen evolution enzyme

Research Project

Project/Area Number 61540490
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionKyushu University

Principal Investigator

YASUSI Yamamoto  Department of Biology, Faculty of Science, Kyushu University, 理学部, 助手 (40091251)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsPhotosynthesis / Photosystem II / Oxygen evolution / Extrinsic proteins / Mn atom / Protein purification / 葉緑体 / 光化学系【II】 / 蛋白質精製
Research Abstract

Photosynthetic oxygen evolution system is closely associated with Photosystem II in chloroplasts. The oxidizing equivalents produced by the photochemical reaction of Photosystem II are accumulated in catalytic Mn atoms and water molecules coordinated with the Mn atoms are oxidized and oxygen is produced.
There are three extrinsic proteins in PS II, and they participate in stabilization of Mn atoms, concentration of Cl^- and binding of Ca^<2+>. In the present study, the organization of the oxygen evolution system was studied by the following ways:
1. An efficient method was developed to purify the three extrinsic proteins from spincach PS II particles where butanol/water phase partitioning and ion-exchange chromatogrphy were employed. The molecular properties of the proteins including N-terminal amino acid sequence were studied with the purified proteins.
2. Organization of the oxygen evolution system was studied by butanol/water phase partitioning of the spinach PS II particles.
3. The amount and stability of the extrinsic 33-kDa protein were studied with thylakoid membranes of Chlamydomonas reinhardi which was grown under Mn-deficient condition. It was suggested from the study that Mn atoms are not involved in the binding of the extrinsic protein and PS II reaction center-binding protein, althouth they are located at the interface between the two proteins.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Yamamoto,Y.: FEBS Lett.195. 155-158 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto,Y.: Arch.Biochem.Biophys.255. 156-161 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto,Y.: Biochim.Biophys.Acta. 893. 579-583 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Isogai,Y.: FEBS Lett.224. 71-74 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto,Y.: J.Biol.Chem.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto,Y.: Biochim.Biophys.Acta. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto, Y., Hermodson, M. A. and Krogmann, D. W.: "Improved purification and N-terminal sequence of the 33-kDa protein in spinach PS II." FEBS Lett.195. 155-158 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto, Y., Nakayama, S., Cohn, C. L. and Krogmann, D. W.: "Highly efficient purification of the 33-, 24-, and 18-kDa proteins in spinach photosystem II by butanol/water phase partitioning and high-performance liquid chromatography." Arch. Biochem. Biophys.255. 156-161 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto, Y. and Kubota, F.: "Specific release of the extrinsic 18-kDa protein from spinach photosystem-II particles by the treatment with NaCl and methanol and its application for large -scale purification of the three extrinsic proteins of photosystem II without chromatography." Biochim. Biophys. Acta. 893. 579-583 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto, Y.: "Organization of the oxygen-evolution enzyme complex studied by butanol/water phase partitioning of spinach photosystem II particles." J. Biol. Chem.in the press (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto, Y.: "Analysis of the relationship between the extrinsic 30-kilodalton protein, manganese and oxygen evolution in the thylakoid of Chlamydomonas reinhardi grown under the manganee-deficient condition." Biochim. Biophys. Acta. in the press (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamamoto,Y.: FEBS Letters. 195. 155-158 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yamamoto,Y.: Proceedings of the Vllth International Congress on Photosynthesis. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yamamoto,Y.: Archives of Biochemistry and Biophysics. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yamamoto,Y.: Analytical Biochemistry.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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