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Mechanisms of expression change and molecular evolution of pepsinogen gene

Research Project

Project/Area Number 61540513
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionFaculty of Science, University of Tokyo

Principal Investigator

YASUGI Sadaoo  Faculty of Science, University of Tokyo, Lecturer, 理学部, 講師 (70011591)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥900,000 (Direct Cost: ¥900,000)
KeywordsPepsinoqens / Vertebrates / Molecular evolution / Ontogenesis / Epithelial-mesenchymal interactions / 上皮-間充織相互作用 / 脊椎動物
Research Abstract

1.Antigens reactive to antibody for adult chicken pepsinogen (ACPg) were found in stomach epithelial cells of all adult vertebrate animals examined. On the other hand, the antigens immunologically related to embryonic chicken pepsinogen (ECPg) exist in epithelial cells of adult fish and ascidians. These observations are important in considering the phylogeny and molecular evolution of vertebrate pepsinogens.
2.ECPg-antigens appear in embryonic or larval period in amphibians and birds, but they are never expressed in mouse. This result, together with the results in 1, seems to suggest the intimate relation between the regulation of ECPg expression in ontogenesis and phylogenesis. We cloned cDNA of mRNA for ECPg and compared the amino acid sequence with those of other pepsinogens, and we found that ECPg is closely related to prochymosin, a pepsinogen which appears in young mammals.
3.To investigate the mechanisms for the regulation of ECPg expression in chicken embryos, we carried out the experimental embryological and molecular biological studies. We found that the epithelial potency to express ECPg and mesenchymal influence on it are equally involved in the process, and that the mesenchymal influence acts on transcription level of ECPg gene expression.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Hayashi,K.(林謙介): J.Biochem.(in press). 103. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takiguchi,K.(瀧口恵子): Roux´s Arch.Develop.Biol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takiguchi,K.(瀧口恵子): Develop.Growth & Differ.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 八杉貞雄: 遺伝. 42. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 八杉貞雄: "消化器の器官形成と組織間相互作用.器官形成研究会編「器官形成-発生生物学から臓器工学まで-」" 培風館, 296 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mizuno,T: "Formation de glandes et expression de pepsinogene dnas l'endoderme du gesier sous l'influence du mesenchyme proventriculaire chez l'embryon de Poulet." C.R.Soc. Biol. 180. 113-116 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takiguchi,K.: "Gizzaad epithelium of chick embryos can express embryonic pepsinogen antigen, a marker protein of proventriculus." Roux's Arch. Develop. Biol.195. 475-483 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yasugi,S.: "Immunological relationships among embryonic and adult chicken pepsinogens: A study with monoclonal and polyclonal antibodies." Develop. Growth & Differ.29. 85-91 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yasugi,S.: "Pepsinogen-like immunoreactivity among vertebrates: Occurrence of common antigenicity to an anti-chicken pepsinogen antiserum in stomach gland cells of vertebrates." Comp. Biochem. Physiol.86B. 675-680 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yasugi, S.: "The structure and expression of globin genes. An example of study of molecular evolution of proteins. (In Japanese)" Seibutsu Kyoiku (Biological Education). 27. 163-166 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yasugi,S.: "Evolution des pepsinogenes au cour du developpement, chez la Souris." C. R. Soc. Biol.181. 450-453 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hayashi,K.: "Molecular cloning and the nucleotide sequence of cDNA for embryonic chicken pepsinogen: Phylogenetic relationship with chymosin." J. Biochem.103. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takiguchi, K.: "Influence of proventricular and gizzard mesenchyme on the organ-specific differentiation of the stomach epithelium of chick embryos." Roux's Arch. Develop. Biol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takiguchi, K.: "Pepsinogen induction in chick stomach epithelia by reaggregated proventricular mesenchymal cells in vitro." Develop. Growth & Differ.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yasugi, S.: "Regulation of gene expression by tissue interactions.(In Japanese)" Iden (Genetics). 42. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yasugi, S.: Organogenesis of digestive organs and tissue interactions. (In Japanese). Baifukan Publishing Co., 102-111 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.Mizuno: C.R.Soc.Biol.180. 113-116 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Takiguchi: Roux's Arch.Dev.Biol.195. 475-483 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Yasugi: Comp.Biochem.Physiol.87. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Yasugi: Comp.Biochem.Physiol.

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Yasugi: Comp.Biochem.Physiol.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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