| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1987: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1986: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
TESTICULAR TERATOMAS IN MICE ARE CONGENITAL ABNORMALITIES ORIGINATING WITHIN SEMINIFEROUS TUBULES IN FETAL TESTES FROM SINGLE PRIMORDIAL GERM CELLS(PGCS). RECENTLY NOGUCHI AND NOGUCHI FOUND THAT THE SINGLE RECESSIVE MUTANT GENE ter CAUSES GERM CELL DEFICIENCY, ACCOMPANIED BY A HIGH INCIDENCE OF TESTICULAR TERATOMAS IN ter HOMOZYGOUS MICE IN 129/Sv-ter STRAIN (JNCI,75,385,1985). IN THIS RESEARCH,THE EXPRESSION OF THE GENE ter IN GERM CELL DEFICIENCY AND TESTICULAR TERATOCARCINOGENESIS WAS ANALYSED FROM THE VIEWPOINT OF DEVELOPMENTAL GENETICS.
1. IN ORDER TO ESTABLISH THE DEVELOPMENTAL BASIS OF STERILITY AND TESTICULAR TERATOCARCINOGENESIS IN ter/ter MICE, THE NUMBER, LOCATION AND MORPHOLOGY OF PGCS WERE EXAMINED IN 8.5-12.5 DAYS EMBRYOS FROM MATING OF +/ter x +/ter AND+/+ x +/+. IT WAS SHOWN THAT THE GENE ter CAUSES EXTRA-GONADAL PGCS DEFICIENCY,NOT ACCOMPANIED BY EXTRACONADAL TERATOCARCINOGENESIS IN ter HOMOZYGOTES IN9.5-12.5 DAYS EMBRYOS IN 129/Sv-ter MICE.
2. IN ORDER TO EXAMINE THE INFLUENCE OF A GENETIC BACKGROUND ON THE ter ACTION, THE GENE ter WAS INTRODUCED ONTO C57BL/6J GENETIC BACKGROUND BY BACKCOSSING FOR 9 GENERATIONS. IT WAS CONCLUDED THAT THE GENE ter ON C57BL/6J GENETIC BACKGROUND IN ter CONGENIC MICE CAUSES GERM CELL DEFICIENCY IN ter HOMOZYGOTES AND THAT GENE(S) OTHER THAN ter IN 129/Sv ARE INVOLVED IN DETERMINING SUSCEPTIBILITY TO TESTICULAR TERATOCARCINOGENESIS IN PGCS.
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