| Project/Area Number |
61550655
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
高分子物性
|
|---|
| Research Institution | Fukui University |
|---|
Principal Investigator |
NAKAMURA Yoshiharu Fukui University, Fac. of Enginnering, Professor, 工学部, 教授 (80020189)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HORI Teruo Fukui University, Fac. of Engineering, Associate Professor, 工学部, 助教授 (90092832)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Project Status |
Completed (Fiscal Year 1987)
|
| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | conjugated redox membrane / intercellular component / histidine-Cu complex / electron transfer / redox enzyme / NADP^+ / NADPH / urethane prepolymer / cyclic voltammetry / 羊毛の細胞間充填物 / 電子移動担体(mediator) / ヒスチジン-銅錯体 / 補酵素NADP / グルタチオンレダクターゼ / グルコース6リン酸デヒドロゲナーゼ |
|---|
| Research Abstract |
An artificial conjugated redox membrane modeled on biological cell membrane was developed. Dehydrogenase and reductase were separately located on both surface sides of the polyurethane hydrogel membrane containing coenzym which transfers electron and proton through the membrane. In order to accelerate the electron transfer across the membrane, the intrecellular component of wool was employed.
Prepolymer prepared from polyethyleneglycol and hexamethylene diisocianate and stabilized with imidazol, was reacted with hexamethylenediamine to get the cationic hydrogel membrane. As redox couple, glucose-6-phosphate dehydrogenase (G6PD)/glucose-6-phosphate(G6P) and glutathione reductase(GRD)/glutathione oxidized form(GSSG) were chosen together with their common common coenzym NADP^+/NADPH.
The conjugated redox reaction proceeded across the NADP^+-adsorbed membrane. On the membrane surface contacted with the G6PD/G6P solution NADP was produced and transfered to the other membrane surface, on which NADPH can drive the other reaction of the GRD/GSSG solution.
The electron transfer effect of the membrane was markedly accelerated by immobilizing the intercellular component extracted from wool into the membrane. From the cyclic voltammograms of a mediator methyl viologen using a bare and a intercellular component modified carbon electrod it was confirmed that the intercellular component could surely act as a good electron transfer.
|
