• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the formation of organelles (peroxisomes) in yeast

Research Project

Project/Area Number 61550723
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 発酵工学
Research InstitutionKyoto University

Principal Investigator

TANAKA Atsuo  Kyoto Univ. Fac. of Eng., Professor, 工学部, 教授 (80026088)

Co-Investigator(Kenkyū-buntansha) SONOMOTO Kenji  Kyoto Univ. Fac. of Eng., Instructor, 工学部, 助手 (10154717)
UEDA Mitsuyoshi Kyoto Un  京都大学, 工学部, 助手 (90183201)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsn-Alkane-utilizable yeast / Candida tropicalis pK 233 / Peroxisomes / Fatty acid <beta>-oxidation system / Bifunctional enzyme / Domain of enoly-CoA hydratese / Genomic DNA sequence for the peroxisomal catalase / 局在化 / 炭化水素資化性酵母 / エノイル-CoAヒドラターゼ / 3-ヒドロキシアシル-CoAデヒドロゲナーゼ / 二頭酵素 / 脂肪酸β-酸化系の進化
Research Abstract

Candida tropicalis pK 233, an n-alkane-utilizable yeast, is an excellent microorganism for studying the localization of specific peroxisomal enzymes in connection with the development of the organelles since development and degradation of peroxisomes as well as the induction and repression of peroxisomal enzymes can be easily controlled by changing the carbon source for growth.
1. Development of the mature peroxisomes was observed during the assimilation of n-alkanes by the yeast. We succeeded in the isolation of the pre-existing peroxisomes from glucose-grown cells and the immature ones from propionate-grown cells as well as the mature ones from alkane-grown cells, and examined their biochemical and cytological properties. The pre-existing peroxisomes form the yeast cells were found to be glyoxysome-like organelles having the key enzymes of glyoxylate cycle.
2. A protein exhibiting only enoyl-CoA hydratase activity was purified from the whole cells of n-alkane-grown cells. On the other hand, a bifunctional enzyme exhibiting enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase activities was obtaind from the same yeast cells when purified in the presence of protease inhibitors. Limited proteolysis of the bifunctional enzyme with <alpha>-chymotrypsin indicated that the former was a part of the bifunctional enzyme. The fact strongly suggests that the domain of enoly-CoA hydratese is separable from the bifunctional enzyme and is noticeable on the evolution of fatty acid <beta>-oxidation system.
3. A clone harbouring the genomic DNA sequence for the peroxisomal catalase has been isolated using the catalase cDNA as a probe, which had been obtained with the antibody-screening method. Analysis of the nucleotide sequence and the deduced amino acid sequence revealed the presence of a noticeable region responsible to the specific localization of catalase into peroxisomes.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Masao Yoshizawa: Agric.Biol.Chem.50. 2637-2638 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Atsuo Tanaka: Ann.N.Y.Acad.Sci.501. 449-453 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirofumi Okada: Agric.Biol.Chem.51. 869-875 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsuyoshi Ueda: Agric.Biol.Chem.51. 2197-2205 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsuyoshi Ueda: FEBS.Lett.220. 31-35 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirofumi Okada: Eur.J.Biochem.170. 105-110 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tatsuo Kurihara: FEBS.Lett.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 植田充美: 蛋白質・核酸・酵素.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 田中渥美: 化学. 42. 808 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masao Yoshizawa: "Some properties of peroxisome-associated D-amino acid oxidase form Candida tropicalis" Agric. Biol. Chem.50. 2637-2638 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Atsuo Tanaka: "Formation of several enzymes associated with alkane utilization by yeast" Ann. N. Y. Acad. Sci.501. 449-453 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirofumi Okada: "Isocitrate lyase and malate synthase of Candida tropicalis grown on differentcarbon sources" Agric. Biol. Chem.51. 869-875 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsuyoshi Ueda: "Relationship between enoyl-CoA hydratase and a peroxisomal bifunctional enzyme, enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase, from an n-alkane-utilizing yeast Candida tropicalis" Agric. Biol. Chem.51. 2197-2205 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsuyoshi Ueda: "Isolation of several cDNA encoding yeast peroxisomal enzymes" FEBS Lett.220. 31-35 (197)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirofumi Okada: "Catalase gene of the yeast Candida tropicalis : Sequence analysis and comparison with peroxisomal and cytosolic catalases form other sources" Eur. J. Biochem.170. 105-110 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tatsuo Kurihara: "Occurrence and possible roles of acetoacetyl-CoA thiolase and 3-ketoacyl-CoA thiolase in peroxisomes of an n-alkane-grown yeast, Candida tropicalis" FEBS Lett.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsuyoshi Ueda: "Peroxisomes" Protein. Nucleic acid. Enzyme.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Atsuo Tanaka: "Peroxisomes with integrated multi-functional and multi-step reactions" Chemistry. 42. 808 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 植田充美: 日本生化学会学会誌. 58(8). 611 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 植田充美: 農芸化学講演要旨集. 62. 76 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Mitsuyoshi Ueda: Agric.Biol.Chem.

    • Related Report
      1986 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi