| Project/Area Number |
61560034
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
園芸・造園学
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| Research Institution | Okayama University |
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Principal Investigator |
MATSUBARA Sachiko Faculty of Agriculture,Okayama University,Professor, 農学部, 教授 (90026378)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Establishment of virus-free plant / Apex culture / Dormancy of apex / Acclimatization / Rooting of shoot / flower disc propagation / garlic / ウィルスフリー株の育成 / 増殖 / 順化 / 組織培養 / 発根培地 / 休眠 / えき芽培養 / 生長点培養 |
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| Research Abstract |
Establishment of virus-free plants, vegetative propagation and acclimatization in garlic and root-promoting treatment of asparagus plantlets cultured in vitro were studied, and the following results were obtained.
Garlic
1. Shoot apexes cultured on an initial medium containing 0.01 mg/1 both of NAA and BA and transferred to a subculture medium containing 0.1 mg/1 NAA and 0.01 mg/1 BA grew vigorously and formed bulblets.
2. Plantlets grown by the method mentioned above were easily acclimatized on rockwool at 20゜C.
3. Virus-free garlic plants were vegetatively propagated by in vitro culture of flower disc cut into 8 explants.
Asparagus
4. Shoots grown from lateral buds rooted effectively by treatment of Rooton (anaphtylacetoamide 0.4 % in mineral powder) at basal parts. Rooting was also promoted by culturing apexes on MS medium containing 0.3 mg/1NAA and 0.1mg/1 BA for 2-3 weeks and transferring to MA medium.
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