• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Genetic analysis of genes in mutants of graminicolous leaf-blight fungi

Research Project

Project/Area Number 61560053
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionKyoto University

Principal Investigator

TSUDA Mitsuya  Faculty of Agriculture Associate Professor, 農学部, 助教授 (10026578)

Co-Investigator(Kenkyū-buntansha) TSUDA Mitsuya  Faculty of Agriculture Associate Professor (10024423)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsTeleomorph / Ascospore / Mutation / Melanin / Cochliobolus hetrostrophus / Leaf-blight / Polyoxin / ネクトリア / アナモルフ-テレオモルフ関係 / ネクトリア病 / ポリオキシン耐性 / メラニン合成 / γ-線 / UV / MNNG / EMS / 子のう胞子分析 / イネごま葉枯病菌 / オフィオボリン / 突然変異株 / ニトロソグアニジン / 分生子
Research Abstract

Crossing experiments with mutants genes, that reveal genetic information about plant pathogenic fungi are essential for elucidationg the nature of these parasites. In this project, I have confirmed the basis for ascospore analysis of graminicolous leaf-blight fungi by using teleomorph formation with mutant genes in vitro.
1. Anamorph-teleomorph connection in some plant pathogenic fungi and its application; Anamorph-teleomorph connection in some plant pathogenic fungi including graminicolous leaf-blight fungi were summarized. Ascocarp-ascospore production under controlled laboratory conditions have demonstrated and compared with conidium production. Ascospore dispersion of the causal agent of Nectria blight of Piper nigrum in the field was also confirmed.
2. Comparison of mutagenes in Cochliobolus heterostrophus mutagenesis; Lethal and mutagenic effects of four kinds of mutagenes (N-methyl-N-nitro-N-nitrosoguanidine(MNNG) ethyl methanesulfonate(EMS), UV light, -ray) on the fungus were studied. MNNG and EMS are almost equally effective in inducing the mutants. The maximum frequency of mutants among the suvaivors was at ca. 5 % survival in MNNG treatment and at 1.4 % survaival in EMS treatment.
3. Genetic analysis of genes in mutants of Cochliobolus heterostrophus; Five types of color mutants were obtained through chemical mutagenesis. The genetic deficient sites of the mutants were determined. The genetic deficient sites of mutants carring Albl,Scy,Brn, and Pgr were at the cyclization of pentaketide, conversion of scytalone, conversion of 1,3,8-THN, and oxidation of 1,8-DHN respectively, in the melanin biosynthesis. The results of crossing experiments indicated that lbl,Alb2 and Brn were closely linked, and that Scy and Pgr were same linkage group. Polyoxin resistant mutant carring Pol was also obtained. Pol was segregated indipendently from above mentioned loci.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Tanaka,C.;Kubo,Y.;Tsuda,M.: Ann.Phytopath.Soc.Japan. 54. 503-509 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamada,M.;Uchida,T.;Tsuda,M.: Ann.Phytopath.Soc.Japan. 54. 303-308 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 獅山慈孝・古澤巌・津田盛也: 植物病害研究. 10. 1-11 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 津田盛也: 植物病害研究. 10. 26-36 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuda,M.;Ueyama,A.: "Some plant pathogenic fungi and their teleomorph in "Pleomorphic fungi:The diversity and its taxonomic implications"" Kodansha;Elsevier., 181-220 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanaka, C.; Kubo, Y.; Tsuda, M.: "Comparison of nutagenes in Cochliobolus hetrostrophus mutagenesis" Ann. Phytopath. Soc. Japan. 54. 503-509 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamada, M.; Uchida, T.; Tsuda, M.: "Ascospore dispersion of the causal agent of Nectria blight of Piper nigrum" Ann. Phytopath. Soc. Japan. 54. 303-308 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shishiyama, J.; Furusawa, I.; Tsuda, M.: "Conidium production in plant pathogenic fungi--- Conidial ontogeny and the effect of light stimulation" Shokubutsu Byogai Kenkyu (Kyoto). 10. 1-11 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuda, M.: "Mechanism of ascospore production---The effect of some factors" Shokubutsu Byogai Kenkyu (Kyoto). 10. 26-36 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuda, M.; Ueyama, A.: Some plant pathogenic fungi and their teleomorphs in "Pleomorphic fungi:The diversity and its taxonomic implications". Kodansha and Elsevier, 181-220 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanaka,C.;Kudo,Y.;Tsuda,M.: Ann.Phytopath.Soc.Japan. 54. 503-509 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hamada,M.;Uchida,T.;Tsuda,M.: Ann.Phytopath.Soc.Japan. 54. 303-308 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 獅山慈孝・古澤巌・津田盛也: 植物病害研究. 10. 1-11 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 津田盛也: 植物病害研究. 10. 26-36 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] C. Tanaka;Y. Kubo, and M. Tsuda: Ann. Phytopath. Soc. Japan. 54. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] M. Hamada;T. Uchida, and M. Tsuda: Ann. Phytopath. Soc. Japan. 54. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] M. Tsuda and A. Ueyama: ""Some plant pathogenic fungi and their teleomorphs" in "Pleomorphic fungi:The diversity and its taxonomic implications"" Kodansha and Elsevier, 19(325) (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 日中千尋,久保康之,津田盛也: 日本植物病理学会報.

    • Related Report
      1986 Annual Research Report
  • [Publications] Tsuda,M.;A.Ueyama: "Pleomorphic fungi:the diversity and its taxonomic implications." Kodansha Ltd./Elsevier,Amsterdam, 181-220 (1987)

    • Related Report
      1986 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi