| Project/Area Number |
61560080
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
NAKAJIMA Tasuku Tohoku Univ. Faculty of Agriculture, Associate Professor., 農学部, 助教授 (20091720)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Yeast / Cell Wall / Yeast mutants / Single cell protein / <beta>-glucan / 細胞壁変異株 |
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| Research Abstract |
Yeast cell wall mutants were obtained by mutagenesis of Saccharomyces cerevisiae X2180-1A, a haploid strain, with N-methyl-N'-nitro-N-nitrosoguanidine.The two S. cerevisiae mutants showed considerable morphological changes and digestibilities to lytic enzymes. Sequential extractions of proteins and carbohydrates from the mutant and wild type cells indicate that the mutants have high protein extractability and lack some wall proteins as well as carbohydrate components.
Chemical structures of cell wall mannans and <beta>-glucans from the mutants were studied. Structural analysis of mutant mannans indicated that both N3 and N3-8 mutant mannans lacked-1,2-mannosyl side chains. Structures of alkali-soluble <beta>-glucans from the mutant cells were also different from that of wild type cells in the ratio of 1,6-linkages to 1,3-linkages. The mutant glucans have much higher ratios of <beta>-1,6-linkages to 1,3-linkages.
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