Studies on the basis of the glucosyltransfer activity of dextransucrase
| Project/Area Number |
61560081
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
KOBAYASHI Mikihiko Faculty of Agriculture, Tohoku University, 農学部, 助手 (00108482)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Dextransucrase / Glucosyltransfer reaction / Chemical modification / Affinity labeling / 多糖生合成 |
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| Research Abstract |
Mechanism of the dextransucrase reaction is important to clarify because of the understanding of the polysaccharide biosynthesis. In this research, we focused our attention on the modification of dextransucrase from Leuconostoc mesenteroides reffering to the change in the activity of glucosyltransfer. The following results were obtained; binding of the substrates, sucrose and dextran, occurred at the separate sites in the dextransucrase molecule for the acceptor and donor molecules. Dextransucrase was inactivated by the modification with water-soluble carbodiimide (EDC) and diethylphro-carbonate. Moreover, an affinity labeling of dextransucrase was conducted by orthophthalaldehyde and epoxide derivative of <alpha>-cyclodextrin (CD-epoxd). Effective inactivation of this enzyme with EDC and CD-epoxd suggested that a carboxyl group was important for the activity. A new function of dextransucrase could be provided by the extensive survey on the modification of respective acceptor- and/or donor-sites of the enzyme.
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Report
(2 results)
Research Products
(10 results)
