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Studies on the basis of the glucosyltransfer activity of dextransucrase

Research Project

Project/Area Number 61560081
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

KOBAYASHI Mikihiko  Faculty of Agriculture, Tohoku University, 農学部, 助手 (00108482)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsDextransucrase / Glucosyltransfer reaction / Chemical modification / Affinity labeling / 多糖生合成
Research Abstract

Mechanism of the dextransucrase reaction is important to clarify because of the understanding of the polysaccharide biosynthesis. In this research, we focused our attention on the modification of dextransucrase from Leuconostoc mesenteroides reffering to the change in the activity of glucosyltransfer. The following results were obtained; binding of the substrates, sucrose and dextran, occurred at the separate sites in the dextransucrase molecule for the acceptor and donor molecules. Dextransucrase was inactivated by the modification with water-soluble carbodiimide (EDC) and diethylphro-carbonate. Moreover, an affinity labeling of dextransucrase was conducted by orthophthalaldehyde and epoxide derivative of <alpha>-cyclodextrin (CD-epoxd). Effective inactivation of this enzyme with EDC and CD-epoxd suggested that a carboxyl group was important for the activity. A new function of dextransucrase could be provided by the extensive survey on the modification of respective acceptor- and/or donor-sites of the enzyme.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report

Research Products

(10 results)

All Other

All Publications (10 results)

  • [Publications] Mikihiko Kobayashi;H.Usugi;K.Matsuda: Agric.Biol.Chem.50. 1051-1053 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mikihiko Kobayashi;I.Yokoyama;K.Matsuda: Agric.Biol.Chem.50. 2585-2590 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mikihiko Kobayashi;H.Usugi;K.Matsuda: Agric.Biol.Chem.51. 2073-2079 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mikihiko Kobayashi, Hideki Uysugi, Kazuo Matsuda: "Intensive UV absorption of dextrans and its application to enzyme reactions." Agric. Biol. Chem.50. 1051-1053 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mikihiko Kobayashi, Ikuko Yokoyama, Kazuo Matsuda: "Substrate binding sites of Leuconostoc mesenteroides dextransucrase evaluated by inhibition kinetics." Agric. Biol. Chem.50. 2585-2590 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mikihiko kobayashi, Hideki Utsugi, Kazuo Matsuda: "Fluorospectral intensity of high-molecular weight dextrans: application to the enzyme reactions." Agric. Biol. Chem.51. 2073-2079 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mikihiko Kobayashi: "Role of carboxyl group of dextransucrase on the catalytic reaction."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mikihiko Kobayashi: Agric.Biol.Chem.50. 1051-1053 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Mikihiko Kobayashi: Agric.Biol.Chem.50. 2585-2590 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Mikihiko Kobayashi: Agric.Biol.Chem.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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