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Structure of Glycinin and Its Significance in Thermal Polymerization of Glycinin Molecule

Research Project

Project/Area Number 61560095
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionKyoto University

Principal Investigator

MORI Tomohiko  Research Institute for Food Science, Kyoto University, 食糧科学研究所, 助教授 (10027188)

Co-Investigator(Kenkyū-buntansha) MATSUMURA Yasuki  Research Instituti for Food Science, Kyoto University, 食糧科学研究所, 助手 (50181756)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsInteraction of glycinin molecule / Structure and Functionality of glycinin / グリシニンの高分子化 / グリシニンのゲル化
Research Abstract

lls globulin (referred to as glycinin), one of the major components of the soybean storage protein, is a multisubunit protein consisting of six subunits in which the acidic and basic polypeptides are linked by disulfide bridges in 1:1 ratio. The glycinin exhibits thermal gelling ability, i.e., polymerization of glycinin molecules. In this study, the correlation between the gelling ability and the protein structure of glycinin was investigated. (1) The constituent subunits of glycinin, ASI,ASII,ASIII, and ASIV, exhibited each characteristic contribution to interactions of glycinin molecules in the course of polymerization. (2) The nucleotide and deduced amino acid sequences of ASI (A_<la>B_x) subunit from G. max var. Shirotsurunoko were determined. The amino acid sequence was compared with those from var. CX635-1-1-1 and Bonminori. The comparison indicated that a considerable degree of homology exists among those subunits, and that homologous regions, variable regions and cysteine residues are located in specific regions of the polypeptide sequence similarly in all the subunits. (3) The interactions of glycinin molecules in the course of polymerization seemed to occur at specific regions of the surface of glycinin molecule through hydrophobic interactions and disulfide exchange reactions. The variable regions and free cysteine residues in polypeptide sequence may be involved in the hydrophobic interaction and disulfide exchange reaction, respectively.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report

Research Products

(15 results)

All Other

All Publications

  • [Publications] Tomohiko Mori: Journal of Agricultural and Food Chemistry. 34. 33-36 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takashi Nakamura: Agricultural and Biological Chemistry. 50. 1287-1293 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takashi Nakamura: Agricultural and Biological Chemistry. 50. 2429-2435 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shigeru Utsumi: Journal of Agricultural and Food Chemistry. 35. 210-214 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shigeru Utsumi: Agricultural and Biological Chemistry. 51. 2139-2144 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tomohiko Mori: Phytochemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tomohiko Mori: "Behavior of intermolecular bond formation in the late stage of heat-induced gelation of glycinin" Journal of Agricultural and Food Chemistry. 34. 33-36 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takashi Nakamura: "Mechanism of heat-induced gelation and gel properties of soybean 7S globulin" Agricultural and Biological Chemistry. 50. 1287-1293 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takashi Nakamura: "Interactions during heat-induced gelation in a mixed system of soybean 7S and lls globulin" Agricultural and Biological Chemistry. 50. 2429-2435 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shigeru Utsumi: "An alternate cDNA encoding glycinin A_<la>B_x subunit" Journal of Agricultural and Food Chemistry. 35. 210-214 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shigeru Utsumi: "Occurrence of dissociable and undissociable soybean glycinin" Agricultural and Biological Chemistry. 51. 2139-2144 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tomohiko Mori: Journal of Agricultural and Food Chemistry. 34. 33-36 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takashi Nakamura: Agricultural and Biological Chemistry. 50. 1287-1293 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takashi Nakamura: Agricultural and Biological Chemistry. 50. 2429-2435 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Shigeru Utsumi: Agricultural and Biological Chemistry.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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