• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Phospholipid Asymmetry of the Biological Membranes and it's Modification

Research Project

Project/Area Number 61560102
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionSaga University

Principal Investigator

YANAGITA Teruyoshi  SAGA UNIVERSITY, 農学部, 助手 (00093980)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Kyosuke  SAGA MEDICAL SCHOOL, 内科, 講師 (00117285)
ENOMOTO Noriyuki  SAGA UNIVERSITY, 農学部, 教授 (20039308)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsLipid asymmetry / Phospholipid biosynthesis / Structure and function of membranes / Regulation of CTP / シチジリルトランスフェラーゼ活性調節 / 生体膜リン脂質 / リン脂質非対称分布 / ペルオキシゾーム誘導剤 / CTP:ホスホコリンシチジリルトランスフェラーゼ / リン脂質合成律速酵素
Research Abstract

The present study was undertaken to understand the factors influencing on the metabolic regulation of phospholipid asymmetry in the biological membranes. At first, we have found that phospholipase C from Cl Welchii and trinitrobenzene sufonate were reasonable probes for the analysis of lipid asymmetry in the biological membranes. By using these probes, we have studied the effects of dietary ingtredients, hormones or drugs on the phospholipid distribution and metabolism of the liver microsomal bilayer in the rat.
From these experiments, it was suugested that the changes in the quantity and quality of microsomal phospholipids by these experimental conditions did not effect on the phospholipid distribution of the microsomal bilayer. THus, phosphatidylcholine distributed predominantly on the cytoplasmic face of the microsomal bilayer and phosphatidylethanolamine distribured predominantly on the lumenal face of the microsomal bilayer. In addition, there were similar molecular species and fatty acid composition of individual phospholipid in each bilayer. However, the distribution of newly synthesized phospholipids was predominantly located on the cytoplasmic face of the bilayer.
Administration of clofibrate or DEHP enhanced the hepatic phospholipid synthesis and increased the activity of microsomal CTP:phosphocholine cytidylyltransferase, the rate-limiting enzyme for phosphatidylcholine biosynthesis. Preferential hydrolysis of phospholipids on the cytoplasmic face of the microsomal membranes decreased the activity of CTP:phosphocholine cytidylyltransferase, suggesting that there is close relationship between the activity of membrane-bound enzyme and the microenvironment of the biological membranes.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] YANAGITA,T.: Biochim. Biophys. Acta. 919. 64-70 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] YANAGITA,T.: Lipids. 22. 572-580 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 柳田晃良: 脂質生化学研究. 29. 277-280 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] YANAGITA,T.: submitted in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] YANAGITA,T.: submitted in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 柳田晃良: 脂質生化学研究. 30. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Teruyoshi YANAGITA: "Di-(2-ethylhexyl) phthalate enhances hepatic phospholipid synthesis in rats" Biochim. Biophys. Acta. 919. 64-70 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Teruyoshi YANAGITA: "Alteration of hepatic phospholipid in rats and mice by feeding di-(2-ethylhexyl)adipate and di-(2-ethylhexyl)phthalate" Lipids. 22. 572-579 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Teruyoshi YANAGITA: "species of Induction and modification of active and inactive CTP:phosphocholine cytidylyltransferase in rats by administration of clofibrate" Proc. J. Conf. Biochem. Lipids. 29. 277-280 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Teruyoshi YANAGITA: "Evaluation of Phospholipase C nad trinitrobenzene sulfonate as probes for phospholipid asymmetry of microsomal membranes"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Teruyoshi YANAGITA: "Phospholipid composition and asymmetry in liver microsomal membranes in rats after administration of peroxisomal proliferators"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.Yanagita;S.Kuzuhara;N.Enomoto: Agric.Biol.Chem.50. 1653-1654 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 柳田晃良,山本匡介,中村武夫,榎本則行: 脂質生化学研究. 28. 49-52 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Yanagita;N.Enomoto;M.Satoh;M.Sugano: Lipids. 22. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Yanagita;M.Satoh;N.Enomoto;M.Sugano: Biochin.Biophys.Acta. (1987)

    • Related Report
      1986 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi