| Project/Area Number |
61560126
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
発酵・醸造
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| Research Institution | Kyushu University |
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Principal Investigator |
HAYASHIDA Shinsaku Faculty of Agriculture, Kyushu University, 農学部, 教授 (50038197)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHINO Sadazo Faculty of Agriculture, Kyushu University, 農学部, 助手 (80117291)
OHTA Kazuyoshi Faculty of Agriculture, Kyushu University, 農学部, 助手 (70112315)
OGATA Seiya Faculty of Agriculture, Kyushu University, 農学部, 教授 (20038277)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Endoglucanase / Cellobiohydrolase / <beta>-glucosidase / Aspergillus / Humicola / Geotrichum / アビセル吸着性セルラーゼ / セルラーゼ / エンドグルカナーゼ / CMCase / 濾紙崩壊力 / 高温糸状菌 |
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| Research Abstract |
The Avicel-adsorbable endoglucanase (MW. 128,000) and the Avicel-unadsorbable endoglucanase (MW. 63,000) were purified from the culture filtrates of mutant No. 140 and mutant no.191, using gel filtration and ion exchange chromatography. Both enzymes were satble to varying pH and temperature and could rapidly decrease the viscosity of CM-cellulose. The Avicel-adsorbable endoglucanase showed intensive filter-paper-disintegrating activity and a highly synergistic effect with the cellulase Preparation of Trichoderma reesei in the hydrolysis of microcrystalline cellulose. This endoglucanase was observed via scanning electron microscopy to disintegrate Avicel fibrils layer by layer from the surface, yielding thin sections with exposed chain ends. The Avicel-unadsorbable endoglucanase showed no disintegrating activity on filter paper and Avicel and a less synergistic effect with the T. reesei cellulase in hydrolyzing microcrystalline cellulose than did the former enzyme. Endoglucanase was therefore divided into two types, Avicel-disintegrating and -nondisintegrating.
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