Polyhydroxyphenols in Japanese brown algae

Research Project

Project/Area Number	61560234
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Fisheries chemistry
Research Institution	HIROSHIMA UNIVERSITY
Principal Investigator	ITO Keiji Faculty of Applied Biological Science, Professor., 生物生産学部, 教授 (30034440)
Co-Investigator(Kenkyū-buntansha)	HORI Kanji Faculty of Applied Biological Science, Assistant Professor, 生物生産学部, 助手 (50116662) MIYAZAWA Keisuke Faculty of Applied Biological Science, Associate Professor, 生物生産学部, 助教授 (20034457)
Project Period (FY)	1986 – 1987
Project Status	Completed (Fiscal Year 1987)
Budget Amount *help	¥2,300,000 (Direct Cost: ¥2,300,000) Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000) Fiscal Year 1986: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywords	brown algae / polyphenol / phloroglucinol / フロログルシノール / 褐藻のポリフェノール
Research Abstract	It was known that brown algae contained tannin-like substances consisting of phloroglucinols. Recently, Glombitza and co-workers have made a series of reports revealing the chemical structure of those in North European brown algae.However, we have a few knowledge on tannin-like substances contained in Japanese brown algae. Therefore, we studied on determination, extraction, fractionation and purification of polyphenolic compounds, in different 11 species of brown algae. These results were as follows: 1. Brown algal polyphenols were determined adequately by measuring of reducing activity with potassium permanganate or Folin-Denis method. The amount of it greatly varied from 0.4 to 5.9% on dry basis. It is pointed out that in Sargassum ringgoldianum and Ecklonia Kurone, the content of phenolic compounds was especialy predominant but low in a species of Genus Sargassum and Myelophycus caespitosus 2. Acetylated brown algal polyphenols gave redish color with both vanillin-H_2 SO_4 and anisaldehyde-H_<> SO_4 reagents. These results suggested that algal polyphenols would be consiting of pyrogallols or phloroglucinols. 3. Toyopearl HW-40S gel column chromatography using methanol as the solvent, gave good results for fractionation and purification of brown algal poly-phenols. 4. From the data of Thin Layer Chromatography, the following results were noticeable. a) Both Ecklonia kurome and E. cava contained free phloroglucinol and 9 kinds of phloroglucinol oligomers including trifuhalol and trifucol. b) on the other hand, Ishige okamurai contained high amount of a dimer of phloroglucinol, difucol, and seven species of brown algae belonging to Genus Sargassum contained higher polymer of phloroglucinol, mainly.