| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1988: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
To elucidate the role of hypothalamic neuropeptides in regulation of anterior pituitary function, Japanese quail, hamster and Japanese longfingered bat were used for immunocytochemical study of distribution of the following neuropeptides in the hypothalamus; luteinizing hormone-releasing hormone (LHRH), somatostatin(SOM), corticotropin-releasing factor(CRF), growth hormone-releasing factor(GRF), arginin vasopressin(AVP), oxytocin (OT), enkephalin(ENK), substance p(SP) and neuropeptide Y.
In the quail, chicken and hamster, LHRH-immunoreactive perikarya were sparsely scattered in the nucleus preopticus(POP) and nucleus septalis medialis (SM), while in the Japanese long-fingered bat, they were scattered throughout the basal hypothalamus, being particularly abundant in the arcuate nucleus, and they project fibers to the internal layer of the median eminence and infundibular stalk. No immunoreactie cells and fibers against anti-cGnRH-II were observed in the hypothalamus and median eminence of the chicken or quail.
SOM-immunoreactive perikarya were widely distributed in the hypothalamus, mainly in the preoptic area, paraventricular nucleus(PVN) and ventromedial and arcuate nuclei. They projected axons to the external layer of the anterior median eminince. SOM perikarya in the PVN of the bat were also reacted to anti-OT serum to suggested probable coexistence of two poptides in one neurons.
In the hamster and Japanese long-fingered bat, CRF-immunoreactice perikarya were distributed in the paraventricular nucleus and medial preoptic area and projected into the external layer of the anterior median eminence, while GRF-immunoreactive perikarya occurred only in the arcuate nucleus and projected into the posterior part of the median eminince.
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