| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
1. Seventeen staphylococcal strains of 14 species and 2 subspecies were exmined for their cell adherence to cell cultures (Vero and MDCK cells), epithelial cells of the nasal cavity, trachea and bladder, and epidermal cells. Epithelial cells of nasal cavity, trachea and bladder were collected from rabbits. Epidermal cells were collected from the skin of mice. Protein A-positive strains (S. aureus Cowan I and S. hyicus subsp. hyicus NCTC 10350) showed remarkably higher rate than protein A-negative (S. epidermidis ATCC 12228, S. saprophyticus ATCC 15305, S.cohnii DSM 20260, S.haemolyticus DSM 20263, S.capitis ATCC 27840, S.sylosus DSM 20266, S.warneri ATCC 27836, S.simulans ATCC 27848, S.hominis ATCC 27844, S.sciuri ATCC 29062, S.saccharolyticus ATCC 14953, S.hyicus subsp. chromogenes NCTC 10530, S.intermedius ATCC 29663&S-35, S.aureus 19-15)of adherence to all cells examined except for the epidermal cells. Among 17 strains, S.sciuri strain was found to adhere best to murine epidermal cells. From these results, it is considered that staphylococcal strains with protein A adhere to various kinds of cells by far more efficiently than strains without protein A.
2. Adherence of protein A-positive (n=3) and -negative strains (n=11) of porcine S.hyicus subsp. hyicus to Vero cells was examined. S.aureus Cowan I strain, which was positive for protein A, was also used. Protein A-positive strains adhered more effectively than did protein A-negative strains. Three strains of S. hyicus subsp. hyicus and S.aureus Cowan I strain which were positive for rotein A showed the great reduction in adhesion after treatment with trypsin. On the other hand, protein A-negative S.hyicus subsp.hyicus strains were unaffected by trypsinization. This suggests that protein A plays a part in adhesion.
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