A new method for measurement of penicilin residues in animal samples by an immobilized penicillinase electrode

Research Project

Project/Area Number	61560356
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Applied veterinary science
Research Institution	Miyazaki University
Principal Investigator	KONDO Fusao Facutly of Agriculture, Miyazaki University, Assistant Professor, 農学部, 助教授 (40145404)
Project Period (FY)	1986 – 1987
Project Status	Completed (Fiscal Year 1987)
Budget Amount *help	¥1,200,000 (Direct Cost: ¥1,200,000) Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000) Fiscal Year 1986: ¥900,000 (Direct Cost: ¥900,000)
Keywords	Penicillin antibiotics / Penicillin sensor / Penicillinase
Research Abstract	A new measurement method for detection of penicillin residues in animal samples by penicillin sensor using penicillinase immobilized on pH electrode has been developed. The following results were obtained. 1. Penicillin-G was most large response in the mV on the enzyme electrode. 2. The electrode response time was in the region of 3 min and the response to penicillin concentration was linear within the range of 6.25 to 2000<micrn>g/ml. The minimum measurable penicillin-G concentration was 6.25<micrn>g/ml. 3. The buffer capacity of the medium influenced the sensitivity of the electrode. At low buffer capacity (0.005 M) the sensitivity of the enzyme electrode to penicillin-G was very high, but the sensitivity of small changes in buffer capacity was relatively large. 4. The new probe had a lifetime of about 2 months. 5. The new penicillin analyzer was shown to be applicable to the rapid assay of peniciliin-G residued in milk and cattle kidney tissues.