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Electron microscopic studies on collagen fibrillogenesis.

Research Project

Project/Area Number 61570009
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionDepartment of Anatomy, Nagoya University School of Medicine

Principal Investigator

KOBAYASHI KUNIHIKO  Dep. Anat., Nagoya Univ. School Med. Assoc. Prof, 医学部, 助教授 (30001051)

Co-Investigator(Kenkyū-buntansha) HOSHINO TAKESHI  Dep. Anat., Nagoya Univ. School Med. Prof., 医学部, 教授 (40000913)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsCollagen / Fubrillogenesis / Electron microscopy / Intermolecular association / negative staining / molecular bundle (SLS) / 4D-staggered linear polymer / テロペプチド / Segment-long-spacing
Research Abstract

1. We previously found that collagen molecules associate at pH 4.5 to specifically form 4D-staggered linear polymers, which are assumed to be the first intermediates in in vitro collagen fibrillogenesis. In this study, we confirmed that the 4D-staggered aggregates (containing various degrees of polymerization) are incorporated into native-type D-periodic fibrils under the physiological conditions. We intended to isolate the oligomers of the different polymericity by gel filtration and to assign the role of each oligomer in collagen fibrillogenesis. The trials were not yet successful.
2. Conditions for electron microscopic identification of the association form of the molecules (dimer) at the first step of collagen self-assembly were investigated and individual methods were optimized. We noticed the direct correlation between negatively stained banding patern of type I collagen molecular bundle (SLS) and the distribution of hydrophobic amino acids along the molecule. The correlation was confirmed with type I and type II collagens.
3. Non-helical parts of the collagen molecule (telopeptide) were revealed to be involved in the 4D-staggered association at pH 4.5.
4. Electron microscopic examination of the sample taken at an early stage of the in vitro fibrillogenesis showed the existance of D-periodic fibrils in addition to thin filaments (preliminaly obsevation).

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] KOBAYASHI,KUNIHIKO: Collagen and Related Research. 6. 195-201 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] KOBAYASHI,KUNIHIKO: Journal of Electron Microscopy. 35. 272-275 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 小林邦彦他: 細胞. 19. 349-353 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 小林邦彦他: 細胞. 19. 379-383 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] KOBAYASHI,KUNIHIKO: Journal of Ultrastructure and Molecular Structure Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] ITO,TAKASHI: Collagen and Realted Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kobayashi, K. et al.: "Incorporation of in vitro formed 4D-staggered linear polymers of collagen into D-periodic fibrils." Collagen and Related Research. 6. 195-201 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kobayashi, K. et al.: "Correlation between negative staining pattern and hydrophobic residues of collagen." Journal of Electron Microscopy. 35. 272-275 (35)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kobayashi, K. et al.: "Bundles of collagen molecules---Electron microscopic atlas. (in Japanese)" The Cell (Saibou in Japanese). 19. 349-353 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kobayashi, K. et al.: "Collagen molecule----a link between biochemistry and ultrastructural morphology. (in Japanese)" The Cell (Saibou in Japanese). 19. 379-383 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kobayashi, K. et al.: "Further evidence for the correlation between the primary structure and the negatively stained banding pattern of collagen molecular bundles." Journal of Ultrastucture and Molecular Structure Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ito, T. et al.: "The effects of protease digestion of telopeptides on the 4D-staggered intermolecular association of collagen." Collagen and Relatee Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] KOBAYASHI,KUNIHIKO: Collagen and Related Research. 6. 195-201 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] KOBAYASHI,KUNIHIKO: Journal of Electron Microscopy. 35(Suppl). 2395-2396 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] KOBAYASHI,KUNIHIKO: Journal of Electron Microscopy. 35(Suppl). 2703-2704 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] KOBAYASHI,KUNIHIKO: Journal of Electron Microscopy. 35. 272-275 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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