| Project/Area Number |
61570011
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Osaka University Medical School |
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Principal Investigator |
ISHIMURA Kazunori Osaka University, associate Professor, 医学部, 助教授 (90112185)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Keisuke Osaka University, associate Professor, 医学部, 助手 (40166666)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Steroid hormones / Adrenal cortex / Ovary / Testis / Immunocytochemistry / Electron microscopy / アロマターゼ / エストロゲン / ATP合成酵素 / 腎髄質間質細胞 / ステロイド17β水酸基脱水素酵素 / 21水酸化酵素 / カルスペクチン / ステロイド3β水酸基脱水素酵素 |
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| Research Abstract |
1.(1) Electron microscopic immunocytochemistry using specific antisera revealed that 3 beta-hydroxysteroid dehydrogenase is present on the membranes of smooth endoplasmic reticulum of the bovine adrenocortical cells. This result indicate that the conversion of pregnenolone to progesterone occurs on the smooth endoplasmic reticulum of the adrenocortical cells. (2) Electron microscopic immunocytochemistry revealed that ATP synthase is present on the inner mitochondrial membranes.
2. Light microscopic immunocytochemistry demonstrated that aromatase (estrogen synthesizing enzyme) is present in the granulosa cells of preovulatory large follicles and in the some cells of corpus luteum, and that 17 betahydroxysteroid dehydrogenase which converts androstenedione to testosterone is present in the cells of theca interna and interstitial glands.
3. Cytochrome p-450 which is involved in the detoxication of some chemical compounds could be localized immunocyotchemically in the steroid-secreting cells of the ovary and testis of 3-methylcholanthrene-treated mice.
4. Calspectin which undercoats the plasma membrane is shown immunocytochemically at the baso-lateral plasma membrane of thyroid follicle epithelial cells of normal rats. In the follicle epithelial cells of TSH-treated rats, calspectin is demonstrated at the apical plasma membrane in addtion to the baso-lateral plasma membrane.
5. Aromatage (estrogen synthesizing enzyme) is demonstrated in the corpus luteum cells of pregnant rats. This result indicate that the corpus luteum cells of the pregnant rats may have the capacity of estrogen synthesis.
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