| Project/Area Number |
61570017
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | Saitama Medical School |
|---|
Principal Investigator |
KANEKO Katsuji Saitama Medical School, Professor, 医学部, 教授 (10049801)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MURATA Eiko Saitama Medical School, Assistant, 医学部, 助手 (40200290)
FUJITA Keiko Saitama Medical School, Assistant, 医学部, 助手 (80173425)
AKITA Masumi Saitama Medical School, Lecturer, 医学部, 講師 (60105905)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Project Status |
Completed (Fiscal Year 1987)
|
| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | Gastric glands / Mucin / Hematoxylin / Mucous neck cells / Neutral mucin / Acidic mucin / 組織化学的研究 |
|---|
| Research Abstract |
Mucous neck cells in the rat stomach which contain neutral mucin were stained with a modified Mayer's hemalum. When alcian blue staining and periodic acid - Schiff(PAS) reaction preceded hematoxylin staining, mucin - containing fundic gland cells could be distinguished in the same tissue sections : surface mucous cells were positive for PAS reaction and / or alcian blue staining, neutral mucin - containing mucous neck cells were stained with hematoxylin, and acidic mucin - containing mucous neck cells were stained with alcian blue. This alcian blue - PAS - hematoxylin staining sequence represents a useful differential staining method for mucins in the gastric mucosa.
Histochemical observation of mucous neck cells of rat employing Mayer's hemalum, alcian blue and high iron diamine showed following distinguishable distribution of mucins depending on physical location of the observed fundic glands. 1. In the region of cardiac glands acidic mucins are predominantly distributed. 2. Along the greater curvature acidic mucins are densely distributed in the upper neck region, while more neutral mucins are observed in the lower neck region. 3. Along the lesser curvature acidic mucins are also observed but not in a regular arrangement seen in the greater curvature. 4. At greater distance from the cardiac glands neutral mucins are predominantly distributed.
|
