Effects of elevated potassium on the survival and differentiation of neurons on culture.
| Project/Area Number |
61570024
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
神経解剖学
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| Research Institution | Shinshu University |
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Principal Investigator |
SHIMIZU Yoshifusa Shinshu University School of Medicine, 医学部, 教授 (80005016)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Y. Shinju School of Alied Medical Sciences, Shinshu University, 医療短期大学部, 講師 (80182921)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | dorsal root ganglion / sympathetic ganglion / spinal cord / tissue culture / elevated potassium / calcium influx inhibitor / neurotransmitter / 急速凍結ディープェッチング法 / 高濃度カリウム培養液 |
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| Research Abstract |
Neurons of the dorsal root ganglion (DRG), sympathetic ganglion (SG) and spinal cord (SC) from chick embryos were cultured in the elevated K^+ medium.
1. In the elevated K^+ medium, survival of both DRG and SG neurons was improved, while that of SC neurons was not improved.
2. In the elevated K^+ medium, the differentiation of DRG neuronswas inhibited, while SG neurons did not become cholinergic and differentiatedadrenergically.
3. The above effects were reduced by addition of Ca^<++> influx inhibitors, Mg^<++> and diltiazem, to the medium.
4. SG neurons differentiated adrenergically in the normal K^+ medium when the SC was co-cultured.
5. In the elevated K^+ medium, movements of DRG and SG neurons at the early stage in culture could be easily recorded and analyzed by the time-lapse cinematography, because the survival of neurons was improved.
6. Using quick-freezing and deep-etching method after removal of cytoplasmic soluble proteins, structures of cytoskeletons and cell organellas of dissociated DRG neurons in culture could be three-dimensionally revealed at high resolution. In teh elevated K^+ medium, neurotubles and neurofilaments tended to form bundles and many vesicles were found on the endoplasmic reticulum.
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Report
(2 results)
Research Products
(8 results)
