| Project/Area Number |
61570031
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
神経解剖学
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| Research Institution | Juntendo University School of Medicine |
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Principal Investigator |
ARAI Yasumasa Professor, Juntendo University School of Medicine, 医学部, 教授 (50053004)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAKAWA Momoko Instructor, Juntendo University School of Medicine, 医学部, 助手 (90103845)
NISHIZUKA Masako Assistant Professor, Juntendo University School of Medicine, 医学部, 講師 (20133332)
MATSUMOTO Akira Assistant Professor, Juntendo University School of Medicine, 医学部, 講師 (80053263)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | neonatal preoptic area / brain transplantation / third ventricle / estrogen / タロシン水酸化酵素 / エストロゲン / 向神経作用 / シナプス形成の促進 / NGF / ガングリオシド / 脳の発達 / シナプス形成 |
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| Research Abstract |
The preoptic area (POA) or cerebral cortex taken from newborn female rats was transplanted into the third ventricle of ovariectomized adult rats. From the day of transplantation, estradiol-17 in a Silastic tube was implanted subcutaneously into host animals for 4 weeks. The POA of cerebral cortes transplants were examined at light- and electron-microscopic levels 4 weeks after transplantation.
Estrogen exposure for 4 weeks via the host induced a significant increase in the volume of the POA grafts. The number of axodendritic shaft and spine synapses was significantly greater in the grafts exposed to estrogen than that of the POA grafts without estrogen tratment. In contrast, there was no significant difference in the volume of conticat tissues transplanted into the brain between the control and estrogentreated grups.
The results suggest that estrogen has a stimulatory effect on the development of neuronal substrates in the ntraventricular POA graft, increasing its volume and synaptic population.
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