| Project/Area Number |
61570040
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
MORITA Yukitomo Hamamatsu University School of Medicine, 医学部, 教授 (80034164)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Katsuhisa Hamamatsu University School of Medicine, 医学部, 助手 (10168693)
TAMOTSU Satoshi Hamamatsu University School of Medicine, 医学部, 助手 (60188448)
SAMEJIMA Michikazu Hamamatsu University School of Medicine, 医学部, 助手 (80135251)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1988: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1987: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Pineal organ / High performance liquid chromatography / Visual pigment / Immunohistochemistry / Anti-opsin / ビジニン抗体 / スペクトル感受性 / 細胞内記録 |
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| Research Abstract |
Photopigments of the direct light-sensitive pineal organ of lamprey, Lampetra japonica, were analyzed by high performance liquid chromatography (HPLC) and by immunohistochemistry.
(1) Retinal and 3-dehydro-retinal were identified by HPLC, that means, the presence of the mixture of vitamin-A1-based pigment and vitamin-A2-based pigment was confirmed.
(2) The ratio of all-trans isomer to 11-cis isomer was higher than that of retina.
(3) Photopigment sensitive to short wavelength light was confirmed by the finding that the ratio of all-trans isomer to 11-cis isomer was reversed when the sample was irradiated by the short wavelength light after the orange light irradiation. The finding coincides the high sensitivity of pineal orgen to short wavelength light demonstrated electrophysiologically.
(4) Spectral sensitivity was measured intracellularly in the pineal photoreceptor cells of adult and larval lamprey. The spectral sensitivity curve of larval pineal has a peak at 505 nm and that of adult was 20 nm shifted toward longer wavelength. This suggests the conversion of the visual pigment during the metamorphosis.
(5) Opsin-positive photoreceptor cells were observed immunohistochemically. The reactivity was restricted in the outer segment of the photoreceptor.
(6) Visinin-positive photoreceptor cells were observed in the periphery of the pineal. Gradation of visinin reactivity suggests the different types of photoreceptors in the pineal.
(4) photoreceptor cells were also confirmed inM parapineal organ immunohistochemically.
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