| Project/Area Number |
61570082
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
環境生理学(含体力医学･栄養生理学)
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
OHNO Hideki Asahikawa Medical College, 医学部, 助教授 (00133819)
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| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Naoyuki Osaka University, 医学部, 教授 (90002188)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1986: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Physical exercise / Permeability of cellular membrane / Liver enzymes / Plasma enzymes / Microsome / <gamma>-Glutamyl transpeptidase / Lysosome / 酵素免疫測定法 / 腎酵素 |
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| Research Abstract |
The purpose of the present study was to investigate the influence of physical exercise on the permeability of the cellular membrane to enzymes. The following three experiments were conducted: 1. Running training: the effects of 10-week physical training (running over 5 km, 6 times/week) on both the activity and concentration of <gamma>-glutamyl transpeptidase (<gamma>GTP) in plasma were investigated on seven sedentary male students. After training the resting plasma <gamma>GTP concentration decreased to half, whereas the <gamma>GTP activity did not vary substantially. This suggests that after training the precursor form of <gamma>GTP did not appear in the plasma and that 'activator protein' appeared in the plasma, possibly due to a leakage from the tissues such as liver. 2. Prolonged exercise: Fifteen male Wistar rats were exercised by swimming until exhausted with a weight equal to 3 % of their body weight in water at 35゜C. Microsomal <gamma>GTP was studied in the liver cell fractions. In vivo and in vitro permeabilities for both <gamma>GTP activity and content were calculated by the following formulas:
In vivo permeability (%)= (extramicrosomal fraction x 100 )/(tramicrosomal fraction + microsomal fraction ) and
in vitro permeability (%) = ( hyposmotic condition )/(osmotic condition ) x 100
The in vivo permeability of both <gamma>GTP activity and content was greatly enhanced after swimming, whereas the in vitro permeability did not change significantly. This indicates that acute exercise increases the permeability of the microsomal membrane of rat liver. 3. Swimming training: Ten male Wister rats were exercised by swimming to 3 h/day, five times/week for 10 weeks in water at 35゜C. After training both the in vivo and in vitro permeabilities-estimated by <gamma>GTP and content-of the membrane of the liver microsomes appeared to reduce, suggesting an increased stability of thr microsomal membrane.
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