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Identification of initiator AUG binding proteins in initiation step of protein synthesis

Research Project

Project/Area Number 61570120
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionNiigata University

Principal Investigator

TAKAHASHI Yoshiaki  Niigata University, Assistant, 医学部, 助手 (60115045)

Co-Investigator(Kenkyū-buntansha) KENMOCHI Naoya  Niigata University, School affairs the faculty, 医学部, 教務職員 (00133124)
MISHIMA Yukio  Niigata University, Lecturer, 医学部, 講師 (30166003)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsInitiation of protein synthesis / Initiator AUG codon / Rabbit reticulocyte lysate / TMV-RNA / 5SRNP / 5SRNP / 80S開始複合体 / 開始AUG配列 / 開始複合体 / リボソーム蛋白 / 紫外線照射 / Ω断片 / 架橋
Research Abstract

An 80S initiation complex was formed by incubation of a rabbit reticulocyte lysate with 5'terminal 70-nucleotide-long fragment of tabbaco mosaic virus RNA (TMV-RNA) without the cap and with its initiator AUG codon at 3'terminus, designated ad ^<32>P<OMEG>, which was labeled with ^<32>pCp. Labeled subfragments of ^<32>P<OMEGA>, which were protected from micrococcal nuclease digestion by 80S initiating ribosomes, were attached to 5SRNA-L5 protein particle (5SRNP). To determine initiator AUG binding proteins, initiation complexes (40S and 80S) were irradiated by UV light to induce crosslinks between ribosomal proteins and ^<32>p<OMEGA>. Analyses of the crosslinked ^<32>p-proteins showed that proteis S7, S10, S25, S29 and L5 in 40S initiation complex, and proteins S7, S25, S29 and L5 in 80S initiation complex. Analyses of a low molecular weight RNA in the 40S complex showed that 5SRNA was stably associated to the 40S subunits. The same result, association of 5SRNA to 40S subunits, was shown in native 40S subunits from liver of rat.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Y.Takahashi: Eur.J.of Biochemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y. Takahashi: "Attachmant of initiator AUG to the 5SRNA L5 protein portion (5SRNP) of the 80S initiation complex." Eur. J. of Biochemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y. Takahashi: "Identifiation of the ribosomal proteins crosslinked to initiator AUG of mRNA within initiation complexes."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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