| Project/Area Number |
61570158
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Sapporo Medical College |
|---|
Principal Investigator |
MINASE Takashi Sapporo Medical College, 医学部, 講師 (00045543)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OYAMADA Masahito Sapporo Medical College, 医学部, 助手 (30183255)
OGAWA Katsuhiko Asahikawa Medical College, 医学部, 教授 (50045514)
|
|---|
| Project Period (FY) |
1986 – 1988
|
| Project Status |
Completed (Fiscal Year 1988)
|
| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1987: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | subplasmalemmal linear density / non-epithelial tumor / ultrastructure / SLD |
|---|
| Research Abstract |
Subplasmalemmal linear densities ( SLDs ) consist of a thin layer of electron-dense material immediately subjacent to the inner leaflet of the palsma membrane. These structures are frequently found in epithelioid cells, macrophages and giant cells in granulomas in the patients of sarcoidosis. Recent work by Mirra and Miles suggested that they were a possible morphologic marker of mesenchymal cells or cells of mesodermal origin.
In this study, we investigated a frequency of SLDs in various tumors and in granulomatous lesions ultrastructurally. SLDs were found 87% of non-epithelial tumors (183/210), 81% of neural tumors (34/42), 100% of granulomatous lesions (16/16), 2% of malignant lymphomas (1/46) but they were not found in any cases of epithelial tumors (0/250). We confirmed that SLDs were a morphologic marker of non-epithelial tumors.
In order to clarify the nature of SLDs, we examined various methods of ultrastructural immunohistochemistry using prolactinoma cells and found that post-embedding methods were the best for this purpose. Among various post-embedding methods, we found that a combination of a new embedding material of LR white or Lowicryl K4M and colloidal gold gave the best result. To investigate SLDs using by immunoelectron microscopy is a continued project.
Since thin sections give only a profile of SLDs, new methods are required to observe a wide area of SLDs. We found that the lysis-squirting method fulfilled for this purpose. Using this method, to observe cells which have SLDs in cultured condition is another continued project.
|
