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Studies on the roles of collagens in cell proliferation and differentiation, tissue organization, and pathological changes

Research Project

Project/Area Number 61570165
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionInstitute of Medical Science, University of Tokyo

Principal Investigator

MATSUZAW akio  Institute of Medical Science, University of Tokyo, 医科学研究所, 助教授 (50012745)

Co-Investigator(Kenkyū-buntansha) TANAKA Shin  Institute of Medical Science, University of Tokyo, 医科学研究所, 教務職員 (70134624)
KIMURA Mikio  Institute of Medical Science, University of Tokyo, 医科学研究所, 助手 (90114462)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsBasement membrane / type IV collagen / mice / kidney / early development / hereditary cataract / lens / autoimmune disease / hormone-dependent mammary tumor / 進展 / 自律性獲得 / 転移 / 【IV】型コラーゲン / マウス腎臓 / マウス初期発生 / コンパクション
Research Abstract

Type IV collagen was isolated and purified from mouse kidneys and the antiserum against it was raised in rabbits. Immunofluorescence and immunohistochemical staining methods were established and applied to research into the roles of the collagen in early or preimplantation development of mice, cataractogenesis in hereditary cataract mice, pathological changes of the kidney in autoimmune mice and progression toward more malignant states of mouse mammary tumors. The specific inhibitor of collagen synthesis, cis-4-hydroxyproline, suppressed compaction of mouse embryos in culture in support of the important role of collagens in morphogenesis in early development. Participation of type IV collagen in this phenomenon is under observation by immunohistochemistry. No evidence has been obtained to indicate a significant role of type IV collagen in lens opacification in hereditary congenic catract mice, BALB/c-nct/nct. Graft-versus-host reaction and hereditary CBA/KI autoimmune mice were used as models for investigation of renal pathogenesis. Immunohistochemical studies with the antiserum against type IV collagen demonstrated that the basement membranes became thicker and more fragile along with pathological changes of the kidney in these models. This is in accord with the disturbed renal dysfunction documental by proteinuria. The amounts of extracellular matrices and type IV collagen, and the collagen-producing capability reduced with progression of hormone-dependent mouse mammary tumor, TPDMT-4, to autonomous and further to metastatic states, suggesting the significant role of collagens in tumor progression.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Oikawa,T.;Iwaguchi,T.;Kimura,M.and Matsuzawa,A.: Chem.Pharm.Bull.34. 789-797 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Matsuzawa,A.;Hayakawa,T.;Takitani,S.and Iwaguchi,T.: Endocrinology. 120. 2346-2356 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kimura,M.and Gleichman,D.: Clin.Immunol.Immunopathol.43. 97-109 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kimura,M.;Ida,S.;Shimada,K.and Kanai,Y.: Clin.Exp.Immunol.69. 385-393 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Matsuzawa,A.and Wada,E.: Exp.Eye Res.submitted.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 田中慎,松沢昭雄: 実験動物. 37. 89-91 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Matsuzawa,A.: "Hormone dependence and independence of mammary tumors in mice,in International Review of Cytology 103." Academic Press,New York,USA., 38 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Oikawa,T., Iwaguchi,T., Kimura,M. and Matsuzawa,A.: "Purification and characterization of type IV collagen from mouse kidney." Chem. Pharm. Bull.34. 789-979 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Matsuzawa,A., Hayakawa,T., Takitani,S. and Iwaguchi,T.: "Comparative studies on estrogen receptors between a pregnancy-dependent mouse mammary tumor (TPDMT-4) and related autonomous tumor." Endocrinology. 120. 2346-2356 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kimura,M. and Gleichman,D.: "Depressed antibody response to exogenous antigens in mice with lupus-like graft-vs-host reaction." Clin. Immunol. Immunopathol.43. 97-109 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kimura,M., Ida,S., Shimada,K. and Kanai,Y.: "Specificity of anti-nuclear antibodies induced in F mice undergoing the graft-vs-host reaction: isotypes and cross reactivities." Clin. Exp. Immunol.69. 385-393 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Matsuzawa,A. and Wada,E.: "Retarded and distinct progress of lens opacification in congenic cataract mice, BALB/c-nct/nct." Exp. Eye Research. Submitted.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Matsuzawa,A.: Hormone dependence and independence of mammary tumors in mice, in International Review of Cytology 103.Academic Press, 38 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tsutomu Oikawa;Takao Iwaguchi;Mikio Kimura;Akio Matsuzawa: Chemical and Pharmacological Bulletin. 34. 789-797 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Akio Matsuzawa: International Review of Cytology. 103. 303-340 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Akio Matsuzawa;Tsuyoshi Hayakawa;Takao Iwaguchi;Shoji Takitani: Endocrinology. 119 Jun【e!~】. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tsutomu Oikawa;Akio Matsuzawa;Takao Iwaguchi: British Journal of Medicine. 54. 91-96 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 松沢昭雄: 日本臨床. 44. 289-294 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 松沢昭雄,武田泰隆: 病態生理. 5. 482-484 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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