| Project/Area Number |
61570166
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Kanazawa University |
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Principal Investigator |
MIZUKAMI Yuji Kanazawa University Hospital, Pathology Section, 医学部附属病院・病理部, 講師 (60110540)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Takuma Kanazawa University Hostpital, Central Clinical Laboratory, 医学部附属病院・検査部, 助教授 (10124710)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | TSH-receptor / Ras-oncogene product / EGF / EGF-receptor / TBII / ras-P21 / 免疫電顕 |
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| Research Abstract |
We have attempted to produce an antibody for human TSH-receptor. Using this antibody, distribution and changes in distribution of TSH-receptor was examined. First, we attempted to purify the TSH-receptor antibody using the serum of patients with high titer of TSH-receptor antibody. The serum containing high titer of TSH-receptor antibody and porcine thyroid membrane fraction were incubated. The binding IgG was released by acidic buffer and released IgG was further purified with IgG affinity chromatography. Using this IgG the localization of TSH-receptor was examined in human thyroid follicular cells. The positive staining was observed at the apical plasma membrane. The only one band was found at Mol. wt 130,000 using the immunoblotting. We also examined the ultrastructural localization of ras p21 product, which participates on GTP regulation of adenylate cyclase. Similar finding that ras p21 product was localized at apical cell membrane ws obtained. Epidermal growth factor (EGF) and EGF-receptor were also examined immunohistochemically in human thyroid. These combined evidences strongly suggested that TSH-receptor is restricted at apical plasma membrane of thyroid follicular cells. The changes in distribution of TSH-receptro in various thyroid diseases have been studied at present.
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