| Project/Area Number |
61570178
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Kumamoto University Medical School |
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Principal Investigator |
HIRASHIMA MITSUOMI Kumamoto University Medical School・ Associate Professor, 医学部, 助教授 (70109700)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | chemotactic lymphokine / monokine / eosinophil / macrophage / 炎症反応 / 遊走性リンカホイン |
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| Research Abstract |
The purpose of this project is to clarify the mechanism of selective regulation of inflammatory lymphokines, such as chemotactic factor and chemotactic inhibitory factor in allergic inflammations. Type of the cells infiltrating into inflammatory lesions is determined by an adjuvant used in the sensitization. For example, macrophages and lymphocytes are predominantly observed in allergic lesions if guinea pigs were sensitized with the antigen in Reund's complete adjuvant, whereas eosinophils are rarely obsered in the lesions. On the other hand, eosinophil accumulation is potentiated by treatment with aluminium hydroxy gel (Alum) or Bordetella pertussis vaccine. In vitro experiments reveal that spleen T lymphocytes of CFA-treated animals produce an eosinophil chemotactic inhibitory factor (ECIF), which selectively inhibits chemotactic response of eosinophils to a T lymphocyte-derived ECE, without any aditional stimulation by 2 weeks after CFA treatment. Production of ECIF is induced by m
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acrophage products of CFA-treated animals.
T lymphocytes of Alum-treated animals produce much potent CFA-treated or adjuvant-nontreated animals. This potentiation of ECF production is mediated by macrophage products (ECF-potentiating factor, ECF-PF) of Alum-treated animals. T lymphocytes require similataneous stimulation of ECF-PF and antigen or mitogen. It is thus suggested that macrophage is involved in the selective regulation of T lymphocyte-derived ECF and also ECIF production in guinea pig system.
T. lymphocyte-derived ECF production is also analyzed in patients with Kimura's disease and with hypereosinophilic syndrome. Peripheral and tissue OKT-4 positive T lymphocytes of those patients produce ECF activity without any additional stimulation. ECF production by the patient T lymphocytes is induced by an adherent cell-derived factor (monocyte-derived factor, MDF) from the patients. No mitogen stimulation is required for ECF production. These results suggested that monocytes and macrophages are involved in the selective regulation of chemotactic lymphokine production. Such project is supposed to be a good tool for clarification of macrophage function in qualitative regulation for cell response in inflammation. Less
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