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Transplantation of isolated hepatocytes in the liver of analbuminemic rats

Research Project

Project/Area Number 61570179
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionAsahikawa Medical College (1988)
Sapporo Medical University (1986-1987)

Principal Investigator

OGAWA Katsuhiro  Asahikawa Medical College, Professor, 医学部, 教授 (50045514)

Co-Investigator(Kenkyū-buntansha) OYAMADA Masahito  Sapporo Medical College, Assistant, 医学部, 助手 (30183255)
榎本 克彦  札幌医科大学, 医学部, 助手 (20151988)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1988: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1987: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsAlbumin / Analbuminemic Rat / Isolated Hepatocytes / 肝内移植
Research Abstract

Transplantation of isolated hepatocytes in the part of the body will be applicable to the therapy of patients with severe liver damage or genetic metabolic disorders. Although the hepatocytes have been reported to be transplantable in the spleen, subcutaneous tissue, intraocular space, etc., the liver is considered to be the most suitable place for transplantation because hepatotrophic factors present in the portal flow will provide appropriate environment for the hepatocytes. In this project, we transplanted normal hepatocytes within the liver of analbuminemic rats and investigated the fate of transplanted hepatocytes by immunohistochemical staining of rat albumin.
Hepatocytes were isolated from the liver of SB rats by collagenase perfusion method. Then 2 x 10^6 hepatocytes were infused into the portal vein of NAR which had received 2/3 partial hepatectomy. Various periods after infusion, the liver was perfusion-fixed and immunostained using an anti rat-albumin antibody.
At 2h, although the most infused hepatocytes were aggregated within the portal vein, a small number of hepatocytes were observed within the sinusoids. These cells were nearly spherical in shape and poorly attached to the sinusoidal wall. At 6h, the transplanted hepatocytes partly attaached to the host hepatocytes where the cytoplasmic processes of the sinusoidal endothelial cells were partly deleted. At 12h, the transplanted cells were completely incorporated within the host hepatic cords and organized within the host hepatic tissue.
The results showed that the hepatocytes infused in the portal vein are readily incorporated and organized within the host liver.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Ogawa,K.,;Yokota,k.,;Mori,M.;Nagase,S.: Proc.Xlth Int.Cong.on Electron Microscopy. 11. 2933-2934 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yokota,K.,;Ogawa,K.,;Mori,M.;Nagase,S.;Sato,K.: Jpn.J.Cancer Res. (Gann). 78. 109-112 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa,K.,;Narasaki,M.;Mori,M.: Toxiclo.Pathol.15. 69-72 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yokata,K.,;Ogawa,K.,;Mori,M.,;Nagase,S.: Cancer Res.48. 389-392 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuji,S.,;Ogawa,K.,;Takasaska,H.,;Sonoda,T.;Mori,M.: Jpn.J.Cancer Res. (Gann). 79. 148-151 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa,K.,;Yokota,K.;Sonoda,T.,;Piao,Z.S.;Mori,M.;Nagase,S.: Int.J.Gancer. 41. 727-731 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa, K.; Yokota, K.; Mori, M.; Nagase, S.: "Settlement of transplanted hepatocytes within the liver of analbuminemic rats." Proc. XIth Int. Cong. on Electron Microscopy. 11. 2933-2934 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yokota, K.; Ogawa, K.; Mori, M., Nagase, S.; Sato, K.: "Transplantation of putative preneoplastic hepatocytes of analbuminemic rats in the livers of Sprague-Dawley x analbuminemic hybrid rats." Jpn. J. Cancer Res. (Gann). 78. 109-112 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa, K.; Narasaki, M.; Mori, M.: "Immunochemical studies of microsomal membranes of rat preneoplastic hepatocytes." Toxicol. Pathol.15. 69-72 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yokota, K.; Ogawa, K., Mori, M. Nagase, S.: "Lack of albumin as a genotypic marker of preneoplastic analbuminemic rat hepatocytes transplanted within albumin-positive liver." Cancer Res.48. 389-392 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa, K.; Tsuji, S.; Yokota, K.; Ikeda, K.; Mori, M.: "Utilization of mosaic animal for the studies of hepatic carcinogenesis." J. Toxicol. Pathol.79. 61-66 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa, K.; Yokota, K.; Sonoda, T.; Piao, Z. S. Mori, M.; Nagase, S.: "Albumin negative hepatocytes in Sprague-Dawley x analbuminemic F1 rats treated with hepatic carcinogens." Int. J. Cancer. 41. 727-731 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogawa,K.;Yokota,K.;Mori,M.;Ngase,S: Proc.Xlth lnt.Cong.on Electron Microscopy. 11. 2933-2934 (1986)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yokota,K.;Ogawa,K.;Mori,M.;Ngase,S.;Sato,K.: Jpn.J.Cancer Res.(Gann). 78. 109-112 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ogawa,K.;Narasaki,M.;Mori,M.: Toxicol.Pathol.15. 69-72 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yokota,K.;Ogawa,K.;Mori,M.;Ngase,S.: Cancer Res.48. 389-392 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsuji,S.;Ogawa,K.;Takasaka,H.;Sonoda,T.;Mori,M.: Jpn.J.Cancer Res.(Gann). 79. 148-151 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ogawa,K.;Yokota,K.;Sonoda,T.;Piao,Z.S.;Mori,M.;Ngase,S.: Int.J.Cancer. 41. 727-731 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ogawa, K. ;Yokota, K. ;Mori, M. and Nagase, S.: Proc. XIth Int. Cong. on Electron Microscopy. 11. 2933-2934 (1986)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yokota, K. ;Ogawa, K. ;Mori, M.;Nagase, S. and Sato, K.: Jpn. J. Cancer REs. (Gann). 78. 109-112 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ogawa, K. ;Narasaki, M. and Mori, M.: Toxicol. Pathol.15. 69-72 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yokota, k. ;Ogawa, K. ;Mori, M. and Nagase, S.: Cancer Res.48. 389-392 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Tsuji, S. ;Ogawa, K. ;Takasaka, H. and Sonoda, T. ;Mori, M.: Jpn. J. Cancer Res. (Gann).

    • Related Report
      1987 Annual Research Report
  • [Publications] Ogawa, K. ;Yokota, K. ;Sonoda, T. ;Piao, Z. S. ;Mori, M. and Nagase, S.: Int. J. Cancer. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ogawa,K.;Yokota,K.;Mori,M.;Nagase,S.: Proc.【XI】th Int.Cong.on Electron Microscopy. 11. 2933-2934 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Ogawa,K.;Yokota,K.;Tsuji,S.;Takasaka,H.;Mori,M.;Nagase,S.: Proceeding of American Association of Cancer Res.28. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 小川勝洋,横田欽一,森道夫,長瀬すみ: 日本病理学会誌. 75. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yokota,K.;Ogawa,K.;Mori,M.;Nagase,S.;Sato,K.: Jpn.J.Cancer Res.(Gann). 78. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 横田欽一,小川勝洋: 札幌医誌. 56. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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