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Establishment of recipient cells for oncogene detection and analysis of transformation-associated cell surface antigen expression

Research Project

Project/Area Number 61570180
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionSapporo Medical University

Principal Investigator

SATO Noriyuki  Department of Pathology, Sapporo Medical College , Associate Professor, 医学部, 助教授 (50158937)

Co-Investigator(Kenkyū-buntansha) OYAMADA Masahito  Department of Pathology, Sapporo Medical College , Senior Instructor, 医学部, 助手 (30183255)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsoncogenes / WFB / tumor antigens / natural killer target structures / heat shock protein / natural killer target structures / 癌遺伝子 / NK標的分子 / 熱ショック蛋白 / 癌抗原 / トランスフォーメーション / 単クローン抗体 / NK活性 / 標的分子 / DNAトランスフェクション
Research Abstract

The detail of anti-tumor surveillance mechanisms by the hosts is still unknown. Surface phenotypes and the ontogeny of NK cells have been widely studied, and there is considerable heterogeneity among NK cell populations. However, little is known about the target structures of tumor cells that are recognized by NK cells. It is also essential to investigate how these structures could be associated with the cell transformation process.
We have demonstrated two different candidates of the NK target molecules, namely 86 kd and 62 kd protein composed of a single polypeptide chain. These were defined by monoclonal antibodies (mabs), #109 and #061, respectively, and were highly associated with the activated H-ras oncogene-induced transformation of WKA rat fetus-derived fibroblast WFB. It is also strongly suggested that the expression of 86 kd and not that of the 62 kd molecule was enhanced on nontumorigenic WFD cells which were stimulated by PDGF. The data suggest that these two different molecules are directly involved in the NK-target cell interactions as the target strucutres. These molecules may play an important role in the antitumor surveillance by the hosts.
On the other hand, another mab #067 recognizing 67 kd single polypeptide reacted selectively with W31 but not with w14 and other EKras-WFB or PyMt-WFB transformants as well as parental WFB. The expression of this antigen was enhanced on W31 by treatment with heat, superoxide and cAMP or cholera toxin. Furthermore, it was induced by these treatment on W14 and other EKras WFB transformants that did not show constitutively this antigen under the regular culture conditions. These data suggested that this antigen is a novel heat-inducible molecule, and might be a tumor specific antigen of WFB cells.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Sato,N.;Yagihashi,A.;Okubo,M.;Torigoe,T.;Takahashi,S.;Sato,T.;Kikuchi,K.: Cancer Research. 47. 3147-3151 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sato,T.;Sato,N.;Takahashi,S.;Okubo,M.;Yagihashi,A.;Torigoe,T.;Takahashi,N.;Okazaki,M.;Asaishi,K.;Kikuchi,K.: Cancer Research. 48. 3892-3897 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Okubo,M.;Yagihashi,A.;Torigoe,T.;Konno,A.;Sato,N.;Kikuchi,K.: Jpn.J.Cancer Res.(GANN). 79. 850-856 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sato,N.;Yagihashi,A.;Torigoe,T.;Okubo,M.;Konno,A.;Takahashi,N.;yamashita,T.;Fujinaga,K.;Kazumaki,N.;Kikuchi,K.: Cancer Research. 48. 2798-2804 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sato,N.;Kikuchi,K.: Gann Monoger.Cancer Res.34. 59-68 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Konno,A.;Sato,N.;Yagihashi,A.;Torigoe,T.;Torimoto,K.;Hara,I.;Wada,Y.;Okubo,M.;Takahashi,N.;Kikuchi,K.: Cancer Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 浜岡利之、橋本嘉幸: "癌と免疫" メジカルビュー社, 176 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 林秀男、山村雄一: "最新免疫研究法" 医学書院, 357 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sato, N.; Yagihashi, A.; Okubo, M.; Torigoe, T.; Takahashi, S.; Sato, T.; Kikuchi,K.: "Characterization of tumor rejection antigen molecules of chemically induced murine colon tumor C-C26." Cancer Research. 47. 3147-3151 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.; Sato, N.; Takahashi, S.; Okubo, M.; Yagihashi, A.; Torigoe, T.; Takahashi, N.; Okazaki, M.; Asaishi, K.; Kikuchi, K.: "Characterization of n-butyl alcohol solubilized, breast tumor specific antigens recognized by a human autologous cytotoxic clone." Cancer Research. 48. 3892-3897 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Okubo, M.; Yagihashi, A.; Torigoe, T.; Konno, A.; Sato, N.; Kikuchi, K.: "The expression of tumor rejection antigen of rat fetus fibroblast transformed by the ras oncogenes." Jpn. J. Cancer Res. (GANN). 79. 850-856 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sato, N.; Yagihashi, A.; Torigoe, T.; Okubo, M.; Konno, A.; Takahashi, N.; Yamashita, T.; Fujinaga, K.; Kuzumaki, N.; Kikuchi, K.: "Identification of the transformation-associated cell surface antigen expressed on the rat fetus-derived fibroblast." Cancer Research. 48. 2798-2804 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sato. N.; Kikuchi, K.: "Isolation and characterization of n-butanol solubilized tumor specific transplantation antigen (TSTA) of chemically-induced mouse colon tumor." Gann Monogr. Cancer Ras.34. 59-68 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sato,N.;Yagihashi,A;Okubo,M.;Torigoe,T.;Takahashi,S.;Sato,T.;Kikuchi,K.: Cancer Research. 47. 3147-3151 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sato,T.;Sato,N.;Takahashi,S.;Okubo,M.,;Yagihashi,A.;Torigoe,T.;Takahashi,N.;Okazaki,M.;Asaishi,K.;Kikuchi,K.: Cancer Research. 48. 3892-3897 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Okubo,M.;Yagihashi,A.;Torigoe,T.;Konno,A.;Sato,N.;Kikuchi,K.: Jpn,J.Cancer Res.(GANN). 79. 850-856 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sato,N.;Yagihashi,A.;Torigoe,T.;Okubo,M.;Konno,A.;Takahashi,N.;Yamashita,T.;Fujinaga,K.;Kuzumaki,N.;Kikuchi,K.: Cancer.Research. 48. 2798-2804 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sato,N.;Kikuchi,K.: Gann Monogr.Cancer Res.34. 59-68 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Konno,A.;Sato,N.;Yagihashi,A.;Torigoe,T.;Torimoto,K.;Hara,I.;Wada,Y.;Okubo,M.;Takahashi,N.;Kikuchi,K.: Gancer Research.

    • Related Report
      1988 Annual Research Report
  • [Publications] 浜岡利之、橋本嘉幸: "癌と免疫" メジカルビュー社, 1-176 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 林秀男、山村雄一: "最新免疫研究法" 医学書院, 1-357 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sato, N.: JNCI. 78. 307-310 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sato, N.: Cancer Res.47. 3147-3135 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yagihashi, A.: Cancer Res.(1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sato, N.: Tumor Res.(1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sato, N.: Gann Monograph. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Okubo, M.: Jap. J. Cancer Res.(1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sato,N.: J.N.C.I.74. 883-888 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] Sato,N.: Cancer research. 46. 362-366 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Sato,T.: Cancer Research. 46. 4384-4389 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Sato,N.: Jpn.J.Cancer Res.(GANN). 77. 473-479 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Sato,N.: J.Nat.Cancer Inst.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Sato,N.: (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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