| Project/Area Number |
61570197
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
寄生虫学(含医用動物学）
|
|---|
| Research Institution | Osaka City University |
|---|
Principal Investigator |
TANABE Kazuyuki OSAKA CITY UNIVERSITY MEDICAL SCHOOL RESEARCH ASSOCIATE, 医学部, 助手 (40047410)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAGAYAMA Katsuhiro OSAKA CITY UNIVERSITY MEDICAL SCHOOL LECTURER, 医学部, 講師 (30047047)
KIMATA Isao OSAKA CITY UNIVERSITY MEDICAL SCHOOL RESEARCH ASSOCIATE, 医学部, 助手 (20153174)
TAKADA Suehisa OSAKA CITY UNIVERSITY MEDICAL SCHOOL PROFESSOR, 医学部, 教授 (10046815)
|
|---|
| Project Period (FY) |
1986 – 1988
|
| Project Status |
Completed (Fiscal Year 1988)
|
| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | MALARIA PARASITES / PLASMODIUM / ERYTHROCYTES / PARASITIC ADAPTATION / GLUCOSE / GENE / クローニング / プラスモティウム |
|---|
| Research Abstract |
To obtain fundamental knowledge necessary for developing malaria vaccines and new antimalarials, mechanisms for adaptation by malaria parasites to their host erythrocytes have been studied at the molecular and cellular levels. Results are as follows:
I. Membrane transport of metabolites in malaria-infected cells. (1) Plasmodium falciparum grows well in human erythrocytes, whose potassium levels ae reduced greatly by treatment with ouabain. (2) An appearance of small pores in the membranes of P. yoelii-infected cells lead to increase in the uptake of 2-deoxy-D-glucose. An electrical potential of protons across the plasma membrane of intraerythrocytic parasites is coupled to an uphill active uptake of 2-deoxy-D-glucose. (3) The mitochondrion of P. yoelii has an inside negative membrane potential.
II. Polymorphism of a surface antigen of P. falciparum merozoite. (1) The gene for a precursor (p190) to major merozoite surface antigens of P. falciparum has been cloned and sequenced. Comparisons of the p190 gene from several parasite isolates has revealed both conserved and variable regions. (2) Variations in the sequence are not widely polymorphic but fall into two types. Southern blot analyses of genomic DNA from 14 isolates has shown that the p190 gene is dimorphic alles. it is suggested that intragenic recombination of the p190 alles creates the antigen's polymorphism.
III. Calcium metabolism of malaria-infected erythrocytes. (1) Disturbance of calcium metabolism of P. yoelii- and P. falciparum-infected cells by Ca^<2+> ionophore, calcium antagonists and calmodulin inhibitors results in the death of the parasites.
|
