| Project/Area Number |
61570218
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | Tokai University, School of Medicine |
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Principal Investigator |
ATSUSHI OZAWA Department of Mictobiology, Tokai University School of Medicine, 医学部・(微生物学), 教授 (50055638)
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| Co-Investigator(Kenkyū-buntansha) |
KEIICHI WATANABE Department of Pathology, Tokai University School of Medicine, 医学部・(病理学), 教授 (00055865)
NOBUHIKO OHNISHI Department of Microbiology, Tokai University School of Medicine, 医学部・(微生物学), 助手 (30051717)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Continuous floe culture / Clostridium difficile / 無菌マウス / CF培養 / 細胞毒活性作用 |
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| Research Abstract |
An anaerobic continuous flow (CF) culture method was used in order to study the effect of Peprostrptococcus magnus and Streptococcus parvulus, anarobic grampositive cocci which are membeis of intestinal bacterial flora, on growth and cytotoxinactivity of Clostridium difficile. The gtowth- and the cytotoxin activity-patterns of C. difficile in an established CF culture of P.magnus were similar to those of C. difficile alone. On the other hand, in the mixed culture system of C. difficile and S. parvulus, the cytotoxin levels were significatly lower as compared with C.difficile alone in spite of the fact no differences existed between growth of C. difficile in mixed and single culture system. The culture filtrate of P. magnus did not influence the growth and cytotoxin production of C. difficile, nor did that of S. parvulus have any effect on growth of C. difficile in static culture. The cytotoxin activity of C. difficile was, however, suppressed by the culture filtrate of S. parvulus. Furthermore, when P. magnus of S. parvulus was statically cultured in a medium containing cytotoxic culture filtrate of C. difficile, the toxin in the medium was not inactivated.
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