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Pathogenesis of Shigella infection

Research Project

Project/Area Number 61570223
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionThe Institute of Public Health

Principal Investigator

OKAMURA NOBORU  Head of Bacteriology Branch,Department of Microbiology The Institute of Public Health, 衛生微生物学部, 室長 (00111592)

Co-Investigator(Kenkyū-buntansha) YOSHIDA YOKO  Chief Investigator,Department of Microbiology The Institute of Public Health, 衛生微生物学部, 主任研究官 (90083732)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsDysentery / shigella / Form I antigen / Cloning / Lipopolysaccharide / Serum bactericidal action / IgA antibody / monomeric IgA / 単量体IgA / 多量体IgA / ELISA法 / 【I】相抗原
Research Abstract

1.Genetic analysis of virulence factors of Shigella:(1)S.boydii large plasmid; We transferred the large plasmid in S.boydii 5 to the plasmid-cured strains of S.boydii,S.sonnei,and Escherichia coli.The transconjugants we obtained acquired the ability to penetrate tissue culture cells,indicating that a large plasmid in S.boydii is responsible for invasiveness.(2)Cloning of S. sonnei form I antigen genes;We cloned,using a cosmid vector,the DNA fragment which contained the genes encoding the synthesis of form I antigen.We constructed the physical map of plasmid pJK1137(36kb)and obtained transposoninserted mutants.From the anaylysis of insertion mutants,gene(s) encoding form I antigen synthesis was mapped in 2 fragments(ca.13db)which were cut with EcoRI,SalI,and hindII,
2.Host defense mechanisms against Shigella infaction:(1) Susceptibility of Shigella spp.to human serum;Difference among Shigella spp.in susceptibility to human serum was observed among clinical isolates of Shigella strains.Order of susceptibility to human serum was as follows;group A > group B > group C,B6 > group D.Analysis of serum treated with heat (56 c 30 min ,or 50 C 20 min) and of activation of complement by lipopolysaccharides (LPS) from each Shigella sp.suggested that the LPS composition,especially the 0 antigen polysaccharide chains,contributes to the difference among Shigella spp.in susceptibility to human serum.(2)Human serum immue response in shigellosis as determined by ELISA;The sera from dysentery patients showed sighificantly higher antibody titers against Shigella LPS,with IgA and IgM at around 7 days and IgG at around 12 days after onset of the disease,than those from healthy persons.IgA antibodies appeared more specific than IgG and IgM antibodies. Analysis of IgA antibody by sucrose gradient ultracentrifugation suggested that during an early convalescent stage of shigellosis serum IgA antibody response against Shigella LPS is largely polymeric.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Noboru Okamura: Bifidobacteria and Microflora. 5. 51-55 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroko Sagara: Antimicrobial Agents and Chemotherapy. 31. 713-719 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kazuya Takahashi: Microbiology and Immunology. 32. 221-223 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Noboru Okamura: Journal of General Microbiology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岡村登: "感染の成立とその臨床" 日本臨床社, 202 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 中谷林太郎: "細菌学はここまで進んだ" 菜根出版, 464 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] N.Okamura et al.: "Interaction of Shigella with bifidobacteria." Biriobbaceria and Microflora.5. 51-55 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Sagara et al.: "Antimicrobial resistance of Campylobacter jejuni and Campylobacter coli with special reference to plasmid profiles of Japanese clinical isolates." Antimicrobial Agents and Chemotherapy. 31. 713-719 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahashi et al.: "Large plasmid in Shigella boydii is responsible for epithelial cell penetration." Microbiology and Immunology. 322. 221-223 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] N.Okamura et al.: "Difference among Shigella spp.in susceptibility to human serum." Journal of general Microbiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岡村登: 日本細菌学雑誌. 40. 857-882 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] Noboru Okamura;R.Nakaya;H.Yokota;N.Yanai;T.Kawashima: Bifitobacteria and Microflora. 5. 51-55 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 高橋和哉,吉田洋子,岡村登: 日本細菌学雑誌. 42. 378 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡村登 ほか: 感染症学雑誌. 61. 162 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡村登: "感染の成立とその臨床" 日本臨床社, 202 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 蜂須賀養悦 監修.岡村登: "細菌学はここまで進んだ" 菜根出版, 464 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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