The experimental research of the demonstration of drug in the tissue specimen from in toricated animal
| Project/Area Number |
61570286
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | University of Tokyo |
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Principal Investigator |
MAKAIDA Masahiro Univ. of Tokyo, 医学部, 講師 (90010107)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIYAMA Ikuo Univ. of Tokyo, 医学部, 教授 (10082075)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Immunoenzyme technics / Toxicology / Methamphetamine / Secondary amines / Phenobarbital / 蛍光物質 / 顕微蛍光分光測光 / メタンフェタミン / 酵素抗体法 |
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| Research Abstract |
The distribution of the drug, which shows the fluorescence in the tissue sections, was easily detected with fluorescen microscope. The estimation of the drug was performed by microscopic fluorescence spectrophotometory, and analysis was performed with miro-spectro-analyzer (01ympus MMSP-QRF). The distribution of acrinol in the tissue sections from autopsy case was demonstrated as strong and specific fluorescence of scrinol. Especially the nuclei of distal renal tubular epithels and the endothelial cells of glomerulus, proximal renal tubular cells and Kupfer cells showed strong fluorescence and its emission spectrum was identified as the spectrum of acrinol.
The other drugs, for example methamphetamine, amphetamine, phenobarbital, etc. which show no or weak fluorescence in the tissue sections, were clearly demonstrated with immunoenzyme technics, The procedure is following; the tissue specimens were fixed in glutaraldehyde and paraformaldehyde mixture fixative, frozen or paraffin sections were prepared, and immunoenzyme staining was performed with specific anit-sera against the drugs.
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Report
(2 results)
Research Products
(18 results)
