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Study on the polymorphisms of human serum proteins by using the methods of isoelectric focusing and immunoblotting.

Research Project

Project/Area Number 61570293
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionEhime University

Principal Investigator

NISHIMUKAI Hiroaki  Ehime University, Associate Professor, 医学部, 助教授 (00079758)

Co-Investigator(Kenkyū-buntansha) 福田 昌子  大分医科大学, 医学部, 助手 (00156788)
岸田 哲子  大分医科大学, 医学部, 助手 (50136793)
SHINOMIYA Takaaki  Ehime University, Professor (30035406)
SHINOMIYA Kaoru  Ehime University, Assistant Professor (90035759)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsIsoelectric focusing / Immunoblotting / Serum protein variant / Genetic polymorphism / 両性担体(アンフォライト)
Research Abstract

The genetic polymorphisms of human serum proteins were studied by using the methods of isoelectric focusing and immunoblotting. The thickness and the composition of the isoelectric focusing gel (polyacrylamide or agarose), and electrode solution were modified to obtain the good separation of protein bands. An ultrathin-layer (0.1 mm thick) gel was useful for TF, GC and PI subtypings, and a high concentration gel (T=10%, C=3%) gave good separation of PI subtypes (e.g. M1M3 and M2M3).
BF subtypes were detected when neuraminidase-treated serum samples were subjected to isoelectric focusing. The gel (pH 3-10) contained 10% sucrose and 1.5 M urea. The phenotype F was separated into two subtypes, named FA and FB. The distribution of BF subtypes in Japanese patients with IgA nephropathy (IgA-N) or idiopathic membranous nephropathy (IMN) was studied, and the significant association of IMN with BF FA (p<0.05) and that with BF FB (p<0.01) were found.
In the course of the study of C7 polymorphism, Japanese families with members carrying C7 silent allele (C7*QO) were found. C6 types in the family members were also examinined; C7*QO was transmitted from a parent to offsprings as a haplotype C7*QO-C6*B. It seems remarkable that C7*QO may be associated with C6*B.
The genetic polymorphisms of C9 and <beta>1H were investigated. The C9 proteins were separated into several bands in a gel containing urea, but the polymorphism was not found. The <beta>1H proteins were separated into two groups of bands in a gel containing high concentration of urea; an anodal- and a middle-region groups. The polymorphism was found in the middle region band groups, however, the inheritance of the bands was not fully made clear. The analysis of the bands in the anodal region should be done to demonstrate the genetic polymorphism of <beta>1H.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report

Research Products

(12 results)

All Other

All Publications

  • [Publications] H. Nishimukai: Journal of the Anthropological Society of Nippon. 95. 273-274 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Nishimukai: Experimental and Clinical Immunogenetics.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Nishimukai: Human Heredity.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Nishimukai: Journal of the Anthropological Society of Nippon.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Nishimukai: "Plasminogen(PLG), Factor I(IF), Factor B(BF) and C7 polymorphisms in Japanese, Philippinos and Micronesians." Journal of the Anthropological Society of Nippon. 95. 273-274 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Nishimukai: "BF subtypes in Japanese patients with IgA nephropathy and with idiopathic membranous nephropathy." Experimental and Clinical Immunogenetics.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Nishimukai: "Three Japanese families with members carrying C7 silent allele (C7*Q0). - possibility for an association between C7*Q0 and C6*B." Human Heredity.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Nishimukai: "Distribution of 2HS-glycoprotein (HSGA) allotypes in a population sample from Kagoshima." Journal of the Anthropological Society of Nippon.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Nishimukai: Journal of the Anthropological Society of Nippon.

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Nishimukai: Human Heredity.

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Nishimukai: Experimental and Clinical Immunogenetics.

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Nishimukai: Journal of the Anthropological Society of Nippon.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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