| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
The size and action of molecular aggregates in human gallbladder bile and model bile solution was studied with using disporsal ultrafiltration unit, and the following results have been shown.
1. In human gallbladder bile, two third of total biliary bile acid was present in large aggregate fraction (molecular weight: above 10,000), one sixth in middle fraction (M.W.:10,000-1,000) and one sixth in small fraction (M.w.:below 1,000). The number and site of hydroxy group and electric charge of side chain were important factors to determine the size of molecular aggregate. The superiority or inferiority of these factors to determine large aggregates was the following order: bile acid with dihydroxy group>with trihydroxy group, bile acid with<alpha>-site of hydroxy group>with (beta)-site of hydroxy group, and side chain with monoanionic form > with dianionic form.
2. In bile cholesterol and calcium can be dissolved by molecular aggregate. The degree was remarkable in large aggregate fraction, comparing with those in middle and small aggregate fractions. However, the dissolution of calcium by middle and small aggregate fraction gained in importance, with decreasing in total biliary lipid concentration.
3. In bile with low concentration of biliary lipid, a capacity for binding calcium with molecular aggregate (dissolution) decreased, and a risk for formation of insoluble calcium salt increased. Therefore, it was presumed that this factor might be connected with calcification of gallstone. Distribution of size of molecular aggregate in bile with chenodeoxycholic acid treatment was signigicantly different from that in bile with ursodeoxycholic acid treatment. Thus, it wasnoted that mechanism of both treaments for gallstone dissolution could not be identical.
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