| Project/Area Number |
61570335
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
KANEKO Eizo Hamamatsu University School of Medicine, 医学部, 助教授 (10010414)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Michiya Hamamatsu University School of Medicine, 医学部, 教授 (60014031)
山田 正美 浜松医科大学, 医学部, 医員
YAMADA Masami Hamamatsu University School of Medicine
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Rat small intestinal endoplasmic reticulum / Transport of L-alanine and D-gluca / G-6-Pase / Acyl-CoA / monoglyceride acyltransferase |
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| Research Abstract |
The endoplasmic reticulum (ER) -enriched microsomes were obtained from rat intestinal mucosal cells by a subfractionation technique to investigate transporting property of amino acids and glucose. Nacl gradient had no effect on the time course of L-alanine and D-glucose uptakes, and the data suggested that the response to medium osmolarity chamges of these microsomes reflected diffusion or binding to the membrane of thes substrates. also, D-glucose binded to the memlrane than L-alanine The specific activities of G-6-Pase in intestinal ER of mouse and guinea pig were ten-fold higher than that of rat , in which the specific activity of G-6-Pase of ER was not significantly different from the homogenate But Acyl-CoA:monoglyceride acyltransferase activity in the not ERwas five-fold higher than that of homogenate. These results suggeoted that ER of the rat inteotinal epithel:um is not important in transport of amino acid and glucose and that Acyl-GoA:monoglyceride acyltransferase is a satisfactory morker enzyme for ER of the rat small intestine, but G-6-Pase not.
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