| Project/Area Number |
61570365
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YASHIZAWA Yasuyuki University of Tsukuba, 臨床医学系内科, 助教授 (20174914)
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| Co-Investigator(Kenkyū-buntansha) |
OHTSUKA Morio University of Tsukuba, 臨床医学系内科, 講師 (00143173)
HASEGAWA Shizuo University of Tsukuba, 臨床医学系内科, 教授 (90009481)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1987: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Isolated perfused Lung System / Antigen Absorption / Antigen presentation / 過敏性肺炎の消褪 / 肺胞マクロファージ / HLA-DR抗原 / 精製ツベルクリン誘導Tリンパ球増殖反応 / 吸入抗原 / 潅流摘出肺 / 灌流摘出肺 |
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| Research Abstract |
The present study was designed to learn the absorption process of inhaled antigens, the capability of antigen presentation by alveolar macrophage and the immunological observations in a patient with hypersensitivity pneumonitis who has been asymptomatio in spite of continuous exposure to the antigen.
The results indicated that, 1) isolated perfused lung system in which the only route for antigen absorption is the alveolo-capillary membrane maintained the lungs intact for 6 h, 2) the absorption rate of antigens was different depending upon the molecular size, 3) alveolar macrophage were capable of presenting PPD to T lvmphocytes in PPD-induced T lymphocyte proliferation, 4) most alveolar macrophages expressed HLA-DR antigen on their surface membrane. 5) intact HAL-DR antigen was required for antigen presentation. 6) continuous exposure to the antigen resulted in the disappearance of hypersensitivity pneumonitis. 7) antibodies to antigen were found in sera and bronchoalveolar (BAL) fluids even at the asymptomatic, 8) BAL revealed lymphocytosis, predominantly suppressor/cytotoxic T lymphocvtes avan at the asymptomatic stage. 9) The ensuing disappearance of disease was closely associated with the reduction of antigen-induced lymphocyte proliferation.
We coneluded that the complicated mechanisms are involved in the pulmonary immunolors.
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