| Project/Area Number |
61570395
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Okinaka Memorial Institute for Medical Research |
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Principal Investigator |
TAKAGI Akio Chief research, Okinaka Memorial Institute For Medical Research, 主任研究員 (20010142)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKI Makoto Researcher, ditto, 研究員
KOJIMA Susumu Researcher, ditto, 研究員 (10183336)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Sarcoplasmic reticulum / Ca-ATPase / muscular dystrophy / mdx mouse / nervous system / 免疫組織化学 / Ca-ATPase蛋白 / カフェイン / 筋小胞 / Ca-ATPose / Caポンプ蛋白抗体 / 筋拘縮 / スキンドファイバー / 筋ジストロフィ / Caポンプ蛋白 / 抗Caポンプ蛋白抗体 / Ca輸送 / 細胞内pH |
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| Research Abstract |
Ca-ATPase protein was purified from the SR of Human skeletal muscle. A polyclonal antiserum was prepared in white rabbits using the Ca-ATPase protein as an antigen. The antibody identified 100KD protein of the SR on the immunoblot. Immunohistochemical studies were performed on cryostantt sections of various myopathies. In Duchenne muscular dystrophy (DMD), the immunostain of SR Ca-ATPase was much decreased. A similar result was obtained in muscle of mdx mice. Tubular aggregates, intracellar structures, which were observed in periodic paralysis, strongly reacted to the antibody, suggesting their SR origin. In the brain and peripheral nerve of mice, a cross-reacting 100KD protein was not detected. However, weakly reacting 36KD protein was observed in these tissues.
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