| Project/Area Number |
61570437
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
NAKASHIMA Yasuhide UOEH,School of Medicine , Associate professor, 医学部, 助教授 (20038780)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHARA Kazuo UOEH,School of Medicine , Lecturer, 医学部, 助手 (30163306)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | smooth muscle cell / endothelial cells / hyperlipidemia / low density lipoprotein / 酸性ムコ多糖体 / 血管内皮細胞 / 血管平滑筋細胞 / 動脈平滑筋細胞 / 動脈内皮細胞 / 酸性ムコ多糖 / Glycosaminoglycans / Heparin / low density lipoprotein |
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| Research Abstract |
We intended experiments to clarify the interaction between vascular smooth muscle cells (SMC) and endothelial cells (EC) by using in vitro system. Especially, they were investi-gated whether low density lipoproteins (LDLS) obtained from hyperlipidemic patients had the promotional effects for the SMC proliferation and whether extrinsic or intrinsic (produced by EC) glycosaminoglycans (GAGS) could inhibit the SMC proliferation induced by hyper-lipidemic LDL.
Firstly, it was confirmed that extrinsic sulfated GAGs and heparin had the inhibitory effect against the SMC proliferaton in dose-dependent manner. Additionally, sulfated GAGs could inhibit the SMC proliferation induced by hyperlipidemic LDLs.
Secondary, we carried out the experiment that GAGs produced by EC could inhibit the SMC proliferation. Thereafter, the hyperlipidemic LDLs were added the medium for EC.The inhibitory effect of ec reduced after addition of LDLs, perhaps via reduct-ion of GAGs production by EC. now we have the plan to culture the SMC and EC stimulaneous-ly in the same culture system. However, it still has the room to be improved. Additionally, it was investigated clinically whether patients with coronary artery diseases had any features from the aspect of apoplipoproteins or antithrombin III and so on.
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