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Malignant clonal expansion of an NK cell subpopulation andits suppressive effect on the growth of hemopoietic stem cells.

Research Project

Project/Area Number 61570447
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionKanazawa University

Principal Investigator

KOIZUMI Shoichi  Department of pediatrics, Kanazawa university School of Medicine, 医学部附属病院, 講師 (50019973)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsNK cells / LGL cells / Leukemia / Bone marrow / Stem cells / CFU-C / Interferon / IL2 / NK細胞白血病 / Tγ細胞 / リンホカイン / 顆粒球減少 / 骨髄幹細胞
Research Abstract

Natural killer (NK) cells, morphologically indistinguishable from large granular lymphocytes (LGLs), are thought to have an important role in the regulation of hematopoiesis. A clonal leukemic population of NK cells was isolated from a 14-year-old girl. They had a Leu-11^+, Leu-7^- phenotype. Since the NK activity was low but was remarkably augmented by stimulation with recombinant human interleukin 2 (rIL-2), but not interferon- (IFN-<gamma>), this NK subset seems to belong to a premature stage in the maturation of NK cells. Because of severe neutropenia throughout the clinical course, we investigated the effects of this NK subpopulation on the in vitro growth of granulocyte-macrophage colony forming cells (GM-CDC). When this NK subset in the presence of rIL-2 was added to the GM-CFC assay system employing normal bone marrow cells, a remarkable suppression of GM-CFC was demonstrated. Culture supernatants from the rIL-2-stimulated NK cells, which had a high level of IFN-<gamma>, also exhibited a significant suppressive activity. Treatment of the supernatants with a specific antibody against IFN-<gamma> abrogated the inhibitory effect on GM-CFC. These data indicate that this unique subset of premature NK cells in the presence of rIL-2 produceIFN-<gamma> which seems to be involved in the suppression of myelopoietic progenitor cells.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Koizumi S.et al: Blood. 68. 1065-1073 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koizumi S.et al: Acta Haematol Jpn. 51. 81-93 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Fine RL,Koizumi S.et al: J Clin Oncol. 5. 489-495 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 小泉晶一他: 日小血誌. 1. 165-170 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 小泉晶一他: 臨床血液. 28. 1375-1384 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koizumi S.et al: Cancer. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koizumi S. et al.: "Malignant clonal expansion of large granular lymphocytes with a Leull+, Leu7-surface phenotype: In vitro responsiveness of malignant cells to recombinant human interleukin 2." Blood. 68. 1065-1073 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koizumi S. et al.: "Suppression of myeloid hematopoiesis by leukemic natural killer cells bearing a Leull+, Leu7- phynotype: Interferon<gamm> as a possible mediator." Acta Haematol Jpn. 51. 81-93 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Fine RL, Koizumi S. et al.: "Effect of calcium channel blockers on human CFU-GM with cytotoxic drugs." J Clin Oncol. 5. 489-495 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koizumi S. et al.: "Comparison of intermittent or continuous methotrexate plus 6-mercaptopurine in regimens for standard-risk acute lymphoblastic leukemia in childhood (JCCLSG-S11)." Cancer in press. 61. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koizumi S. et al.: Effects of high-dose methotrexate on in vitro growth of hemopoietic progenitor cells. In Methotrexate in cancer therapy. Editors; Kimura K and Wang YM.Raven Press, New York, 49-54 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shoichi Koizumi et.al: Blood. 68. 1065-1073 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Shoichi Koizumi et.al: Caneer Research.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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