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Studies of nephritogenic antigens in human renal basement membranes

Research Project

Project/Area Number 61570478
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionKinki University School of Medicine

Principal Investigator

YOSHIOKA Kazuo  Department of Pediatrics, Kinki University School of Medicine, Lecturer, 医学部小児科, 講師 (60111035)

Co-Investigator(Kenkyū-buntansha) ISEKI Takahiro  Department of Pediatrics, Kinki University School of Medicine, fellow, 医学部小児科, 助手 (30176250)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsGlomerular basement membrane / Tubular basement membrane / Alveolar basement membrane / Anti-basement membrane antibody / Pulmonary hemorrhage / レクチン / 尿細管基底膜 / 抗基底膜抗体 / 基底膜 / ELISA
Research Abstract

1. Analysis of nephritogenic antigens in human alveolar basement membrane (ABM). Goodpastue (DP) antigens, protein components reactive with human anti-glomerular basement membrane (GBM), were identified in human ABM using an enzyme-linked immunoassay, Western blotting and immunoprecipitation. Very cationic 22-28 kD and 40-48 kD components were detected by blot analysis combined with two-deimensional gel electrophoresis. Inhibition ELISA suggested anti-GBM antibodies recognized the same epitops(s) on the GBM and GBM. Heterogeneity of GP antigens in ABM was demonstrated by blotting: monomeric antigens were absent or minimal in ABM of three out of 10 normal individuals. In immunoprecipitation, anti-GBM antisera from patients with pulmonary hemorrhage precipitated GP antigens, byt those from patients without it did not. These data may explain why only some patients with anti-GBM nephritis have lung involvement.
2. Analysis of a target antigen in human anti-tubular basement membrane (TBM) nephritis. Collagenase digestion released antigenic moieties from normal human TBM. Anti-TBM antisera from four patients with anti-TBM nephritis showed on immunoprecipitation 54 kD and 48 kD with pI 7-8 and 6.5-7 antigenic components. Peincubation of normal kidney sections with RCA I, specific to galactose, inhibited the binding of anti-TBM antibody to TBM, suggesting the target antigen is a galactose containing glycoprotein. 54 kD antigen was isolated and purified using gel chromatography, ion exchange column, RCA I affinity column and reverse phase chromatography.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yoshioka,K.: Journal of Immunology. 136. 1654-1660 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 吉岡加寿夫: 日本腎臓学会雑誌. 28. 1321-1328 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka,K.: American Journal of Pathology. 126. 592-598 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka,K: Clinical and Experimental Immunology. 74. 419-426 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka,K: "Recent Advances in Pediatric Nephrology" Elservier Scientific Publishers B.V., 676 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka, K.: "Characterization of tubular besement membrane antigens in human kidney" Journal of Immunology. 136. 1654-1660 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka, K.: "Enzyme immunoassay of anti-renal basement membrane antibodies" Japanese Journal of Nephrology. 28. 1321-1328 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka, K.: "Ricinus communis agglutinin I blocks the binding of human anti- renal basement membrane antibodies to the kidney" American Journal of Pathology. 126. 592-598 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka, K.: "Identification of Goodpasture antigens in human alveolar basement membrane" Clinical and Experimental Immunology. 74. 419-426 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka, K.: "Isolation of a target antigen in human anti-tubular basement membrane nephritis" preparing manuscript.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka, K.: Characterization of nephritogenic antigens in human tubular basement membrane. Elsevier Publishers B.V., 93-97 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshioka,K.;Iseki,T.;Morimoto,Y.;Okada,M.;Eryu,N.;Maki,S.: Clinical and Experimental Immunology. 74. 419-426 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshioka,K.,Okada,M., Takemura, T. & Maki,S.: American Journal of Pathology. 126. 592-598 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yoshioka,K.& Maki,S.: Recent Advances in Prdiatric Nephrology. 93-97 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yoshioka,K.,Iseki,T.,Morimoto,Y.,Matsumoto,K.&Maki,S.: Clinical and Experimental Immunology.

    • Related Report
      1987 Annual Research Report
  • [Publications] Yoshioka,K.;Morimota,Y.;Iseki,T.;Maki,S.: Journal of Immunology. 136. 1654-1660 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 吉岡加寿夫,井碩孝博,岡田満,森本康夫,坂野一美,牧淳: 28. 1321-1328 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yoshioka,K.;Okada,M.;Takemura,T;Maki,S.: American Journal of Pathology.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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