| Project/Area Number |
61570493
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
ICHIHASHI Masamitsu Kobe University School of Medicine, Assistant Professor, 医学部, 助教授 (00030867)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MISHIMA Yutaka Kobe University School of Medicine, Professor, 医学部, 教授 (10073743)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Project Status |
Completed (Fiscal Year 1987)
|
| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | thermal neutron / survival curve / ^<10>B-paraboronophenylalanine / 熱中性子捕捉 / フェニールアラニン欠乏食 / 非競合阻害 / ボロン / B-16黒色腫 |
|---|
| Research Abstract |
Thermal neutron capture therapy (TNCT) represents a promising modality for selective targetting irradiatin of tumor cells. The crucial point of successful use of TNCT for melanoma is how the ^<10>B-compound is selectively incorporated into melanoma cells and accumulate in melanoma tissue after administrations of the chemicals.
Aim: To find a new condition in which ^<10>B_1-paraboronophenylalanine (^<10>B_1-BPA) is incorporated more effectively into melanoma cells in vitro and also invivo than the previous conditions, resulting in a more efficient lethal effect of ^<10>B_1-BPA-TNCT on melanoma cells and melanoma.
Results and Comments: (1)Inactivation of melanoma cells by TNCT increased in dose dependent manner of ^<10>B^1-BPA upto 80 <micrn>g/ml. (2)Inhibitory effect of ^<10>B_1-BPA on the uptake of ^3H-tyrosine using cultured B-16 melanoma cells was shown by Lineweaver-Burke plot. (3)Enhanced lethal effect of TNCT was obtained when melanoma cells were pre incubated with tyrosine and phenylalanine deficient MEM (D-MEM) containg ^<10>B_1-BPA(10-50<micrn>G/ML) compared to that of melanoma cells preincubated with the usual MEM containg the same account of ^<10>B_2-BPA. (4)^<10>B content of B-16 melanoma cells incubated with D-MEM containg 10<micrn>g ^<10>B_1-BPA/ml was 4 times higher than that of usual MEM. (5)Greene's melanoma transplanted in hamster that had been fed with tyrosine and phenylalanie-free diet for 7 days before administartion incorporated 1.2-2.4 times higher ^<10>B than that of hamster that had been fed with usual diet. These results obtained from in vivtro and in vivo experiments strongly subject that tyrosine- and phenylalanine-free diet could inactivate melanoma tissue even in human melanoma irradiated with thermal neutron.
|
