| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
Pemphigus vulgaris (PV) and bullous pemphigoid (BP) are blistering diseases which are chracterized by the production of autoantibodies against PV or BP antigens expressed in the extracellular spaces of epidermal zones, respectively. It has been shown that <gamm>-interferon(<gamm>-IFN), which is one of the lymphokines secreted by T cells, induces or enhances expression of several proteins on the surface of many kinds of cells, including Ia antigens required for the activation as a restriction molecule in T cell recoginition of antigens. We cultured PAM 212 cells, which are from a spontaneously transformed kerationocyte cell line, with <gamm>-IFN and we investigated the effects of <gamm>-IFN on the expression of Ia, PV and BP antigens on the cell surface by using flow cytometry, So as not to dissolve these antigens, we prepared PAM 212 cell suspensions without trypsinization and we stained PAM 212 cells by propidium iodide (PI) which dyes dead cells to be red in order to exclude dead cells in flow cytometry analysis. Then we showed that <gamm>-IFN induced the expression of Ia antigen on PAM 212 cell surface and also enhanced the amounts of PV and BP antigens expressed on it. These findings suggest that <gamm>-IFN released from the activated T lymphocyte, may play a role in the pathogenesis of these autoimmune bullous diseases.
Now we have got PAM 212 cells transfected with <gamm>-IFN gene. Allostimulatory and antigen presenting activities of these <gamm>-IFN gene transfected PAM 212 cells and also their effect on the expression of Ia, PV and BP antigen and on the tumor immunity against the growth of them selves, in other words, on the autoimmunity are going to be in vestigated.
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