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Studies on the mechanism of antidepressants using clonal cell lines.

Research Project

Project/Area Number 61570529
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionSaitama Medical School

Principal Investigator

HIGUCHI Teruhiko  Saitama Medical School, Lecturer, 医学部, 講師 (90105883)

Co-Investigator(Kenkyū-buntansha) EBISAWA Takashi  Saitama Medical School, instructor, 医学部, 助手 (00201369)
WATANABE Yoshifumi  Saitama Medical School, instructor, 医学部, 助手 (90182964)
五十嵐 良雄  埼玉医科大学, 医学部, 助手 (20118623)
山崎 潤  埼玉医科大学, 医学部, 助手 (60182482)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsC6 glioma cells / <beta>-adrenoceptor / cyclic AMP / cyclic AMP / パロキセチン結合 / 再とり込み機構 / 神経系腫瘍細胞 / PC-12細胞 / 【C_6】細胞 / チロシン水酸化酵素活性 / 【β_3】レセプター
Research Abstract

The direct effects of chronic treatment with the anti-depressants on the number of <bet>-adrenoceptors and the accumulation of cyclic-AMP stimulated with isoproterenol were studied on C6 intact cultured cells. The cells were treated chronically for 5 days with 10^<-5> M decipramine (DMI), imipramine (IMI) or mianserin(MIA). Binding studies were performed with the radioligand ^3H-CGP-12177. These treatment did not induce the decrease in the number of <bet>-adrenoceptors nor the change of affinity. On the other hand, chronic exposure (4 days) to DMI or MIA led to a decrease in isoproterenol-induced accumulation of cyclic AMP in a dose dependent manner (10^<-7> -10^<-5> M). When the cells were cultured with haloperidol, the decrease in isoproterenol-induced accumulation of cyclic AMP was found only in the concentration of 10^<-5> M and 5x10^<-6> M. These result suggest that antidepressants (DMI and MIA) directly act on the secondmessenger system without any change of <bet>-adrenoceptors when these drugs were administered chronically. In addition, the concentration of 10^<-6>-10^<-7> M of antidepressants should be used for these kind of experiments because the change of the response of cyclic AMP with 10^<-5>-10^<-6> M was found to be not specific for antidepressants.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report

Research Products

(4 results)

All Other

All Publications (4 results)

  • [Publications] 渡辺義文: 精神医学. 29. 1271-1276 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 樋口輝彦: "神経系培養細胞を用いた抗うつ薬の作用機序に関する研究(第一報)" 昭和62年度厚生省神経疾患研究委託費報告書, (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yoshifumi Watanabe: "Effects of Chronic Antidepressant Treatment on Serotonin Reuptake Mechanism and Paroxetine Binding Sites" CLINICAL PSYCHIATRY. 29. 1271-1276 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 渡辺義文: 精神薬療基金年報 第18集. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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